butyryl-CoA脱氢酶的三维结构Megasphaera elsdenii。
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Djordjevic年代,CP, Stankovich MT JJ
butyryl-CoA脱氢酶的三维结构Megasphaera elsdenii。
生物化学。1995年2月21日,34 (7):2163 - 71。
- PubMed ID
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7857927 (在PubMed]
- 文摘
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butyryl-CoA脱氢酶的晶体结构(BCAD) Megasphaera elsdenii包裹着acetoacetyl-CoA分辨率2.5已经解决了。的酶结晶P422空间群与细胞尺寸= b = 107.76 a和c = 153.67。BCAD是一种细菌模拟的短链酰coa从哺乳动物线粒体脱氢酶。哺乳动物酰coa脱氢酶是黄素腺嘌呤二核苷酸(时尚)含有酶,催化脂肪酸的机会的第一步。虽然特定底物链长度,他们表现出很高的序列同源性。BCAD解决了分子结构的替代方法使用猪的肝脏介质的原子坐标链酰coa脱氢酶(MCAD)。结构精制的r因子为19.3%。的整体多肽折叠BCAD MCAD的相似。E367 BCAD在同一位置和类似的构象MCAD催化基地,E376。主要酶BCAD和MCAD之间的差异是他们的底物特异性和重要的氧气反应由BCAD但不是MCAD展出。 The substrate binding cavity of BCAD is relatively shallow compared to that of MCAD, as consequences of both a single amino acid insertion and differences in the side chains of the helices that make the binding site. The si-face of the FAD in BCAD is more exposed to solvent than that in MCAD. Therefore solvation can stabilize the superoxide anion and considerably increase the rate of oxidation of reduced flavin in the bacterial enzyme.