结构的氧化长链flavodoxin从项圈藻7120 2一项决议。
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Rao圣,Shaffie F,于C, Satyshur KA,仓库管理员BJ, Markley杰,Sundarlingam M
结构的氧化长链flavodoxin从项圈藻7120 2一项决议。
蛋白质科学。1992年11月,1(11):1413 - 27所示。
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1303762 (在PubMed]
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长链的结构从光合藻青菌flavodoxin项圈藻7120 2 A分辨率决定了分子置换法使用原子坐标的长链flavodoxin Anacystis nidulans。第三个长链的结构从陨石球粒flavodoxin管最近报道。的氧化a . 7120 flavodoxin属于单斜晶体空间群P2 (1) = 48.0, = 32.0 b, c = 51.6,和β= 92度,一个分子的不对称单元。2与振荡强度数据收集电影在国际象棋同步源和处理与Rmerg产量9795个独立的强度为0.07。其中,8493年反思我> 2σ和被用于分析。模型得到的分子取代最初使用XPLOR精制的模拟退火程序。重复改装成省略地图和几轮共轭梯度优化导致热阻模型包含原子的0.185蛋白质残基2 - 169,黄素单核苷酸(FMN),和104年溶剂分子。的单核苷酸FMN显示许多相互作用蛋白的异咯嗪环,ribityl糖和5 '磷酸。黄素环有其嘧啶结束埋到蛋白质,和功能都可以访问溶剂二甲苯优势。FMN交互在所有三长链结构是相似的,除了O4 ribityl链的,这与88年用力推的羟基侧链在7120 a,而在其他两个水分子。 The phosphate group interacts with the atoms of the 9-15 loop as well as with NE1 of Trp 57. The N5 atom of flavin interacts with the amide NH of Ile 59 in A. 7120, whereas in A. nidulans it interacts with the amide NH of Val 59 in a similar manner. In C. crispus flavodoxin, N5 forms a hydrogen bond with the side chain hydroxyl group of the equivalent Thr 58. The hydrogen bond distances to the backbone NH groups in the first two flavodoxins are 3.6 A and 3.5 A, respectively, whereas in the third flavodoxin the distance is 3.1 A, close to the normal value. Even though the hydrogen bond distances are long in the first two cases, still they might have significant energy because their microenvironment in the protein is not accessible to solvent.(ABSTRACT TRUNCATED AT 400 WORDS)