隔离、序列分析和转录的研究flavodoxin基因Anacystis nidulans R2。
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Laudenbach DE, Reith我,施特劳斯NA
隔离、序列分析和转录的研究flavodoxin基因Anacystis nidulans R2。
J Bacteriol。1988年1月,170 (1):258 - 65。
- PubMed ID
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3121586 (在PubMed]
- 文摘
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nonheme, iron-sulfur蛋白铁氧还蛋白光合电子传递链的终端组成。铁压力的条件下,许多蓝细菌和真核藻类取代铁氧还蛋白与黄素蛋白flavodoxin。flavodoxin是克隆的基因从藻青菌Anacystis nidulans R2通过使用三个混合寡核苷酸探针源自偏聚球藻属sp.应变PCC 6301氨基酸序列。核苷酸序列分析发现一双513 -基地-开放阅读框,推导出其他长链flavodoxins氨基酸序列具有同源性。假设初始蛋氨酸的蛋白水解乳沟残渣,a的分子量nidulans R2 flavodoxin是18609。印迹杂交条件下减少紧缩只发现一份flavodoxin a . nidulans R2基因组序列。北部(RNA)污点用克隆flavodoxin基因探针杂交分析表明没有检测记录的条件下铁饱和度。然而,在缺铁增长条件下flavodoxin基因似乎是转录作为一个更大的操纵子的一部分。操纵子取得了至少三个记录。第一次是约1100基地(指定的RNA 1)并立即终止上游的5 '末端flavodoxin开放阅读框。 A second, less abundant transcript of approximately 1,900 bases (designated RNA 2) encoded all of RNA 1 as well as the flavodoxin polypeptide. Analysis indicated that both transcripts initiate in close proximity to each other. A third, minor transcript of approximately 1,100 bases (designated RNA 3) was detectable downstream of the flavodoxin gene sequence. Addition of iron-stressed A. nidulans R2 cells resulted in almost total loss of detectable mRNA transcripts within 60 min of the addition. The ferredoxin gene transcript has previously been characterized as a monocistronic message of approximately 430 bases (M. E. Reith, D. E. Laudenbach, and N. A. Straus, J. Bacteriol. 168: 1319-1324, 1986). Here we show that the ferredoxin message is detectable under all iron regimes tested is quantitatively unaffected by decreases in iron availability to the cells.