作业的配体的几何形状和氧化还原电位trihaem ferricytochrome c3从Desulfuromonas acetoxidans。
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Coutinho IB,特纳DL,科斯塔HS Legall J,泽维尔AV
作业的配体的几何形状和氧化还原电位trihaem ferricytochrome c3从Desulfuromonas acetoxidans。
欧元。1997 1月15日,243 (2):474 - 81。
- PubMed ID
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9030775 (在PubMed]
- 文摘
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细胞色素c551.5 trihaem细胞色素的细胞色素c3家庭隔绝Desulfuromonas acetoxidans。虽然有几种x射线结构tetrahaem细胞色素的家庭,没有x射线结构trihaem色素。研究了细胞色素C551.5氧化形成的二维核磁共振的方法。观察到的模式interhaem NOESY连接性与血红素在协议核心结构之前由NMR减少蛋白[Coutinho) i B。、车工、d、L。刘,m . Y。LeGall, j . &泽维尔,a . v .(1996)生物。Inorg。化学1,305 - 311]。之间的相似之处发现血红素细胞色素的核心结构和氨基酸序列c551.5和tetrahaem细胞色素c3允许每个血红素是专门分配的多肽序列,和归因的中点个人血红素氧化还原电位。这还允许个人氧化还原电位是分配给每个血红素在NMR谱。13 c共振的顺磁的变化分析了取代基血红素的π分子轨道摄动D4h对称。 The parameters of this analysis have been shown to be controlled by the orientation of the axial ligands in several other bis-His-coordinated haems and hence the ligand geometry was deduced for cytochrome C551.5. The structural analogy between the relative haem plane orientations in cytochrome c551.5 and the tetrahaem cytochromes c3 is found to extend to the axial ligands with the largest differences being in the vicinity of the deleted fourth haem, using the numbering of cytochrome c3 haems.