血小板衍生生长因子-d在发育和成熟人肾脏中的表达。
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Changsirikulchai S, Hudkins KL, Goodpaster TA, Volpone J, Topouzis S, Gilbertson DG, Alpers CE
血小板衍生生长因子-d在发育和成熟人肾脏中的表达。
肾内科杂志2002;62(6):2043-54。
- PubMed ID
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12427128 (PubMed视图]
- 摘要
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背景:血小板衍生生长因子(PDGF)是一类由硫化物键合二聚体结构组成的生长调节分子。两种被充分研究的PDGF肽(PDGF- a和PDGF- b)已被证明介导广泛的生物效应。PDGF- d是PDGF家族的新成员。PDGF-D基因的初步研究发现其在血管壁细胞中表达，提示其可能参与血管的发育和病理。然而，其在人体肾脏组织中的定位从未被研究过。方法:采用免疫组化方法检测PDGF-D在胎儿(N = 30)和成人(N = 25)肾组织中的表达，采用人源PDGF-D亲和纯化抗体。免疫肽的抗体吸收证实了该抗体的特异性。Western blotting和逆转录聚合酶链反应(RT-PCR)也证实了PDGF-D蛋白和基因在人肾脏中的表达。结果:在发育中的肾脏中，PDGF-D首先在发育中的肾元逗号形和s形结构的上皮细胞中表达，在肾小球分化后期的内脏上皮细胞中表达最为一致。此外，PDGF-D可在发育中的肾盂间质间充质细胞和胎儿动脉血管平滑肌细胞中发现。 In the adult normal kidney, PDGF-D was expressed by the visceral epithelial cells. There was persistent expression in arterial smooth muscle cells as well as in some neointimal smooth muscle cells of arteriosclerotic vessels, and expression in smooth muscle cells of vasa rectae in the medulla. PDGF-D could be identified at the basolateral membrane of some injured tubules in areas of chronic tubulointerstitial injury routinely encountered in aging kidneys. Western blotting of homogenates of adult kidneys demonstrated monospecific bands at 50 kD corresponding to previously established size parameter for this protein. RT-PCR of human kidney RNA resulted in a 918 basepair band, the sequence of which corresponded to human PDGF-D (Genbank number AF336376). CONCLUSIONS: To our knowledge, these are the first studies to localize PDGF-D in human kidneys and suggest that PDGF-D may have a role in kidney development. PDGF-D was shown to bind to PDGF beta receptor, which localizes to mesangial cells, parietal epithelial cells, and interstitial fibroblasts, suggesting potential paracrine interactions between those cells and the visceral epithelium.