人类和啮齿动物肠脂肪酸结合蛋白基因。比较分析的结构、表达和联动关系。
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糖果哒,Birkenmeier呃,Klisak IJ, Zollman年代,火花RS,莫汉达斯·T,卢西AJ,戈登霁
人类和啮齿动物肠脂肪酸结合蛋白基因。比较分析的结构、表达和联动关系。
J生物化学杂志。1987年11月25日,262 (33):16060 - 71。
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2824476 (在PubMed]
- 文摘
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肠脂肪酸结合蛋白(I-FABP)据信参与吸收,细胞内代谢和/或肠上皮细胞内运输的长链脂肪酸。15.1 -kda啮齿动物蛋白质低先生是一个家庭的成员已经进化到细胞质蛋白质绑定不同的配体。我们已经决定基因的核苷酸序列编码人类I-FABP并定义其蛋白质的一级结构的产品。人类基因I-FABP跨越3382个核苷酸,包含4个外显子(103或128、173、108和312个碱基对)。打断了3个内含子(1194、1023和444个碱基对)。132 -残渣老鼠和人类I-FABPs有82%的氨基酸序列的身份。污点的杂交研究rna准备各种各样的成年猕猴组织以及人类肠道和肝脏表明I-FABP信使rna是局限于肠道。I-FABP mRNA没有检测到人类enterocyte-like细胞株的培养,这表明它可能是一个敏感的分化的标志,villus-associated,小肠粘膜细胞。相似的老鼠和人类基因的组织表达表现出,我们比较他们的5 ' nontranscribed地区。最优比对两个序列的身份在260年公布64%核苷酸5 '立即开始转录。 Matrix plots revealed a 14-nucleotide long repeated sequence (5'-TGAACTTTGAACTT-3') in the 5' nontranscribed region of both genes as well as in a comparable region of another family member that is expressed in enterocytes, cellular retinol binding protein II. The linkage relationships between I-FABP and the homologous liver FABP (L-FABP) gene were defined in mice and humans. The mouse genes were mapped using restriction fragment length polymorphisms and recombinant inbred strains. The I-FABP gene is located on mouse chromosome 3 between the amylase 1,2 (Amy 1,2) and alcohol dehydrogenase 3 (Adh-3) loci while the L-FABP gene is on mouse chromosome 6 within 3 centimorgans of the lymphocyte antigen-2 (Ly-2) locus. Mouse L-FABP may be identical to the major liver protein-1 (Lvp-1) which is encoded by a gene situated within a centimorgan of Ly-2. Human gene mapping studies were carried out using a panel of mouse-human somatic cell hybrid clones as well as in situ hybridization to metaphase chromosomes.(ABSTRACT TRUNCATED AT 400 WORDS)