突触传递的烟碱乙酰胆碱受体鼠海马organotypic文化和切片。
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Hefft年代,穆勒Hulo年代,伯特兰D, D
突触传递的烟碱乙酰胆碱受体鼠海马organotypic文化和切片。
杂志。1999年3月15日,515 (Pt 3): 769 - 76。
- PubMed ID
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10066903 (在PubMed]
- 文摘
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1。全细胞夹记录传入刺激复合突触电流引起的谢弗络脉表明NMDA封锁,AMPA GABAA受体介导和组件6-nitro-7-sulphamoyl -苯并(f) quinoxaline-2 3-dione (NBQX), 3 - ((R) 2-carboxypiperazine-4-yl) propyl-1-phosphonate (R-CPP)和苦味毒,分别留下了一个小的剩余电流在39的41 CA1锥体神经元organotypic文化和9的16 CA1敏锐地准备细胞片。2。当前2表示。9 + / - 0.4%的化合物诱发突触反应organoypic文化在切片和1.4 + / - 0.5%。它凭借略整流电流-电压曲线和逆转3.4 + / - 5的潜力。1 mV。3所示。这种剩余电流对GABAB受体阻滞剂,purinergic,毒蕈碱的5-HT3受体,但它本质上是被烟碱受体拮抗剂d-tubocurarine(91 + / - 4%封锁;20 microM),部分被alpha-bungarotoxin (200 nM)和methyllycaconitine(10海里),两个对手更高的选择性alpha7 subunit-containing烟碱受体(48 + / - 3%,55 + / - 11%封锁,分别)。 4. The residual current was of synaptic origin, since it occurred after a small delay; its amplitude depended upon the stimulation intensity and it was calcium dependent and blocked by the sodium channel antagonist tetrodotoxin. 5. We conclude that afferent stimulation applied in the stratum radiatum evokes in some hippocampal neurones a small synaptic current mediated by activation of neuronal nicotinic receptors.