识别和描述第二个邻氨基苯甲酸盐合成酶基因的铜绿假单胞菌:互换性两邻氨基苯甲酸盐合成酶和演化的影响。
文章的细节
-
引用
复制到剪贴板 -
Essar DW,希伯L, Hadero,克劳福德IP
识别和描述第二个邻氨基苯甲酸盐合成酶基因的铜绿假单胞菌:互换性两邻氨基苯甲酸盐合成酶和演化的影响。
J Bacteriol。1990年2月,172 (2):884 - 900。
- PubMed ID
-
2153661 (在PubMed]
- 文摘
-
两个邻氨基苯甲酸盐合成酶基因对已确定在铜绿假单胞菌。他们克隆,测序,灭活体外抗生素抗性基因的插入,并返回铜绿假单胞菌,取代野生型基因。一个邻氨基苯甲酸盐合成酶的酶参与色氨酸合成;其基因是指定trpE和trpG。邻氨基苯甲酸盐合成酶的酶,编码phnA和phnB参与绿脓菌素的合成,吩嗪色素的生物特征。trpE和trpG独立转录;从假单胞菌同源基因已经被克隆putida。吩嗪通路基因phnA和phnB cotranscribed。克隆的phnA phnB基因对补充trpE和trpE (G)大肠杆菌的突变体。同源基因中没有p putida PPG1, non-phenazine生产商。 Surprisingly, PhnA and PhnB are more closely related to E. coli TrpE and TrpG than to Pseudomonas TrpE and TrpG, whereas Pseudomonas TrpE and TrpG are more closely related to E. coli PabB and PabA than to E. coli TrpE and TrpG. We replaced the wild-type trpE on the P. aeruginosa chromosome with a mutant form having a considerable portion of its coding sequence deleted and replaced by a tetracycline resistance gene cassette. This resulted in tryptophan auxotrophy; however, spontaneous tryptophan-independent revertants appeared at a frequency of 10(-5) to 10(6). The anthranilate synthase of these revertants is not feedback inhibited by tryptophan, suggesting that it arises from PhnAB. phnA mutants retain a low level of pyocyanin production. Introduction of an inactivated trpE gene into a phnA mutant abolished residual pyocyanin production, suggesting that the trpE trpG gene products are capable of providing some anthranilate for pyocyanin synthesis.