CobT纯化和表征,nicotinate-mononucleotide: 5, 6-dimethylbenzimidazole phosphoribosyltransferase酶从鼠伤寒沙门氏菌LT2。

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Trzebiatowski JR Escalante-Semerena JC

CobT纯化和表征,nicotinate-mononucleotide: 5, 6-dimethylbenzimidazole phosphoribosyltransferase酶从鼠伤寒沙门氏菌LT2。

生物化学杂志。1997年7月11日;272 (28):17662 - 7。

PubMed ID

9211916 (在PubMed

]

文摘

我们报告的净化和生化特征氰钴胺素生物合成的酶nicotinate-mononucleotide: 5, 6-dimethylbenzimidazole phosphoribosyltransferase (CobT)鼠伤寒沙门氏菌。cobT是过表达和纯化的蛋白质大约97%的同质性。NH2-terminal序列分析证实,被cobT编码的蛋白质纯化。齐次CobT催化合成的N1 - (5-phospho-alpha-D-ribosyl) 5, 6-dimethylbenzimidazole。高效液相的身份chromatography-purified产品被快原子轰击质谱证实。CobT活动45度优化的C和pH值10.0。烟酸的明显公里单核苷酸是680 microM;明显的为5公里,6-dimethylbenzimidazole小于10 microM。CobT使用烟酰胺单核苷酸磷酸核糖捐赠。CobT phosphoribosylated替代基地基板包括苯并咪唑4 5-dimethyl-1, 2-phenylenediamine,咪唑,组氨酸,腺嘌呤和鸟嘌呤体外。 The resulting ribotides were incorporated into cobamides that were differentially utilized by methionine synthase (EC 2.1.1.13), ethanolamine ammonia-lyase (EC 4.3.1.7), and 1,2-propanediol dehydratase (EC 4.2.1.28) in vivo. The lack of base substrate specificity by CobT may explain the inability to isolate mutants blocked in the synthesis of 5, 6-dimethylbenzimidazole in this bacterium.

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药物靶点

药物	目标	类	生物	药理作用	行动
腺嘌呤	Nicotinate-nucleotide——dimethylbenzimidazole phosphoribosyltransferase	蛋白质	鼠伤寒沙门氏菌(应变LT2 / SGSC1412 /写明ATCC 700720)	未知的	不可用	细节