核苷酸序列的afimbrial-adhesin-encoding afa-3基因簇及其易位通过侧翼IS1插入序列。
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加西亚MI, Labigne Le Bouguenec C
核苷酸序列的afimbrial-adhesin-encoding afa-3基因簇及其易位通过侧翼IS1插入序列。
J Bacteriol。1994年12月,176(24):7601 - 13所示。
- PubMed ID
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8002584 (在PubMed]
- 文摘
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阿发基因簇编码afimbrial丰富(afa)表达的uropathogenic diarrhea-associated大肠杆菌菌株。的plasmid-borne afa-3的生物合成基因簇负责AFA-III adhesin属于博士的血凝素家族。报道在这工作是9.2 kb的核苷酸序列重组质粒pILL61插入,它包含afa-3基因集群从cystitis-associated大肠杆菌克隆菌株(故事本来)。afa-3基因集群包含六个开放阅读框,显示指定afaA afaF。它是在两个不同组织的转录单位。阿发的六个方面中的五个显示显著的同源蛋白质编码的产品前所述粘附系统允许我们给他们每个人一个假定的函数属性的生物起源AFA-III adhesin。AfaE被确认为结构性adhesin产品,长期以来而AfaB和我们被认为周质的伴侣和外膜锚定蛋白,分别。AfaA和AfaF产品被证明是同源PapI-PapB转录调控蛋白。没有函数可以归因于AfaD产品,以前显示的基因合成功能性adhesin可有可无的。上游的afa-3基因簇,一个1.2 kb地区被发现96%相同的RepFIB序列的一个产肠毒素的大肠杆菌质粒(P307),说明质粒共同祖先。 This region contains an integrase-like gene (int). Sequence analysis revealed the presence of an IS1 element between the int gene and the afa-3 gene cluster. Two other IS1 elements were detected and located in the vicinity of the afa-3 gene cluster by hybridization experiments. The afa-3 gene cluster was therefore found to be flanked by two IS1 elements in direct orientation and two in opposite orientations. The afa-3 gene cluster, flanked by two directly oriented IS1 elements, was shown to translocate from a recombinant plasmid to the E. coli chromosome. This translocation event occurred via IS1-specific recombination mediated by a recA-independent mechanism.