基本转录翻译组件的组织和表达基因在极高温真细菌Thermotoga maritima。
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廖D,丹尼斯PP
基本转录翻译组件的组织和表达基因在极高温真细菌Thermotoga maritima。
J生物化学杂志。1992年11月15日,267 (32):22787 - 97。
- PubMed ID
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1429627 (在PubMed]
- 文摘
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长5789 -核苷酸EcoRI片段基因组的Thermotoga maritima,被不断化解双方的交互融合,L1, L10,从大肠杆菌和L12核糖体蛋白基因序列,克隆和测序。片段编码5转运rna (tRNA (met1), 8月反密码子互补;tRNA (met2), 8月;tRNA(刺),ACA;UAC tRNA(酪氨酸);tRNA (trp), UGG)转录nusG termination-antitermination因素,四个50 S亚基核糖体蛋白质不断化解,L1, L10、L12,伴部分RNA聚合酶β亚基的蛋白质。五个tRNA基因,nusG基因,不断化解,L1, L10、L12核糖体蛋白基因组成一个复杂的转录单位。记录似乎开始从上游启动子,P1,位于前面的tRNA (met1)从三个内部基因和启动子:P2位于前立即tRNA (met2)基因;PL10附近的开始L1-L10基因间的空间,最后和PL12 L10基因的序列。领袖的tRNA序列是切除区域的P1 -和P2-initiated记录。 Three putative but potentially important regulatory sequences were identified within this operon: an L1 translational control site, a transcription attenuator, and a strong rho-independent terminator. The strong terminator located distal to the L12 gene overlaps a fifth promoter, P beta, which is used to initiate transcripts of the downstream RNA polymerase beta subunit gene. The T. maritima NusG protein exhibits 43% amino acid sequence identity when aligned to the E. coli protein; the alignment is interrupted by a large 171-amino acid-long insertion into the T. maritima protein after codon 45.