功能和分子特征的人类中性溶质aquaporin-9频道。
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莫顿CC Tsukaguchi H, Weremowicz年代,《马
功能和分子特征的人类中性溶质aquaporin-9频道。
杂志。1999年11月,277 (5 Pt 2): f685 - 96。
- PubMed ID
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10564231 (在PubMed]
- 文摘
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在新陈代谢活跃细胞,协调运输水和溶质维持渗透体内平衡是很重要的。我们最近发现了一个广泛的selective-neutral溶质频道,AQP9,从大鼠肝脏,允许通过各种各样的水和中性溶质(h . Tsukaguchi c . Shayakul美国诉伯杰,b·麦肯齐s Devidas w·b·Guggino a . n . van Hoek a和m .《。生物。化学。273:24737 - 24743,1998)。人类相同器官(hAQP9)和76%的氨基酸序列的身份鼠AQP9 (rAQP9)描述,但其渗透率是局限于水和尿素(k . Ishibashi m . Kuwahara顾y, y田中,f . Marumo和佐佐木。物化学。Biophys。Commun >, 244: 268 - 274, 1998)。这里我们报告一个重新评估hAQP9功能特征的,其组织分布,其基因的结构,和它的染色体定位。当非洲爪蟾蜍卵母细胞中表达,hAQP9允许通过各种各样的普通弓箭溶质,包括尿素、多元醇、嘌呤、嘧啶根皮素和mercurial-sensitive方式。 These functional characteristics are similar to those of rAQP9. Based on Northern blot analysis, both rat and human AQP9 are abundantly expressed in liver, whereas, in contrast to rAQP9, hAQP9 is also expressed in peripheral leukocytes and in tissues that accumulate leukocytes, such as lung, spleen, and bone marrow. The human AQP9 gene is composed of 6 exons and 5 introns distributed over approximately approximately 25 kb. The gene organization is strikingly similar to that reported for human AQP3 and AQP7, suggesting their evolution from a common ancestral gene. The promoter region contains putative tonicity and glucocorticoid-responsive elements, suggesting that AQP9 may be regulated by osmolality and catabolism. Fluorescence in situ hybridization assigned its locus to chromosome 15 q22.1-22.2. Our data show that hAQP9 serves as a promiscuous solute channel expressed in both liver and peripheral leukocytes, where it is ideally suited to transport of metabolites and/or nutrients into and out of these cells