监管的C代谢L-methionine酿酒酵母。

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卤KL,安东尼EA

监管的C代谢L-methionine酿酒酵母。

j . 1972年12月,130 (3):773 - 83。

PubMed ID

4198357 (在PubMed

]

文摘

1。叶酸衍生物的浓度在有氧文化的酿酒酵母(A.T.C.C. 9763)测定微生物鉴定采用干酪乳杆菌(A.T.C.C. 7469)和片球菌属酵母(A.T.C.C. 8081)。细胞培养在媒体缺乏l-methionine包含叶酸衍生物浓度高于细胞生长在同一个媒体补充2.5 mumol l-methionine /毫升。高度共轭的衍生品的浓度也降低了补充与l-methionine生长介质。2。deae纤维素柱层析法提取准备的细胞生长在这些条件下显示的浓度甲基化tetrahydrofolates大大减少了蛋氨酸补充。小浓度的减少也观察到甲酰化和未被取代的衍生品。3所示。四种酶的浓度C(1)代谢比较6 h后的增长的存在和没有l-methionine (2.5 mumol /毫升)。formyltetrahydrofolate合成酶的具体活动,methylenetetrahydrofolate还原酶和丝氨酸hydroxymethyltransferase并没有因为这个而改变治疗但5-methyltetrahydrofolate-homocysteine甲基转移酶是下降了约。 65% when l-methionine was supplied. The activities of 5-methyltetrahydrofolate-homocysteine methyltransferase, serine hydroxymethyltransferase and formyltetrahydrofolate synthetase were not appreciably altered by l-methionine in vitro. In contrast this amino acid was found to inhibit the activity of methylenetetrahydrofolate reductase. 4. Feeding experiments employing sodium [(14)C]formate indicated that cells grown in the presence of exogenous methionine, although having less ability to convert formate into methionine, readily incorporated (14)C into serine and the adenosyl moiety of S-adenosylmethionine. 5. It is suggested that exogenous l-methionine controls C(1) metabolism in Saccharomyces principally by regulation of methyl-group biogenesis within the folate pool.

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药物酶

药物	酶	类	生物	药理作用	行动
蛋氨酸	Methylenetetrahydrofolate还原酶	蛋白质	人类	未知的	抑制剂	细节