隔离和表征人类因素IX的cDNA编码。
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Kurachi K,戴维电子战
隔离和表征人类因素IX的cDNA编码。
《美国国家科学院刊S a . 1982年11月,79 (21):6461 - 4。
- PubMed ID
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6959130 (在PubMed]
- 文摘
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cDNA图书馆准备从人类肝脏已经筛选了IX因子(圣诞因素),凝血因子,参与凝血的中间阶段。图书馆与单链DNA检查准备丰富mRNA的狒狒和第九凝血因子合成寡核苷酸混合物。质粒被确认,包含1466个碱基对的cDNA插入编码人类第九因子。插入两侧是11 g c尾巴和18个碱基对5’和3’端,分别。它还包括138个碱基对,伴领导人代码序列,成熟的蛋白质,1248个碱基对,代码一个终止密码子,48个碱基对3 '非编码序列的结束。领导序列包含46个氨基酸残基,并提出,这个序列包括一个信号序列和序列为成熟的蛋白质,在等离子体中循环。1248个碱基对的代码为416个氨基酸组成的多肽链。这种蛋白质的伴地区包含12个谷氨酸残基中转换为gamma-carboxyglutamic酸成熟的蛋白质。这些谷氨酸残基编码的棉酚和呕吐。arginyl肽债券中裂解转化人类因子IX因子IXa夏被确认为Arg145-Ala146, Arg180-Val181的因素。 The cleavage of these two internal peptide bonds results in the formation of an activation peptide (35 amino acids) and factor IXa, a serine protease composed of a light chain (145 amino acids) and a heavy chain (236 amino acids), and these two chains are held together by a disulfide bond(s). The active site residues including histidine, aspartate, and serine are located in the heavy chain at positions 221, 270, and 366, respectively. These amino acids are homologous with His57, Asp102, and Ser195 in the active site of chymotrypsin. Two potential carbohydrate binding sites (Asn-X-Thr) were identified in the activation peptide, and these were located at Asn157 and Asn167. The homology in the amino acid sequence between human and bovine factor IX was found to be 83%.