低分子量的影响,从胎鼠头顶标准肝素钙的流失。
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肖尼西SG,年轻的E,德尚P, Hirsh J
低分子量的影响,从胎鼠头顶标准肝素钙的流失。
血。1995年8月15日,86 (4):1368 - 73。
- PubMed ID
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7632944 (在PubMed]
- 文摘
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骨质疏松症是一个公认的并发症的长期使用肝素。然而,肝素可以影响骨代谢的机制尚不清楚。我们报告,未分离肝素刺激骨吸收过程中,低分子量肝素(lmwh)的发生率,陈香美,logiparin, ardeparin产生明显不如未分离肝素钙流失。评估从骨钙流失,我们量化45 ca的释放到胎鼠头顶的培养基。45 ca释放是剂量依赖性的方式增加了额外的未分离肝素或lmwh;但是高出50倍LMWH浓度被要求获得同等效果依诺肝素。因此,在浓度> = 2微克/毫升(0.35 anti-Xa单位/毫升),依诺肝素刺激45 ca release 1.53 + / - 0.06折。45 ca释放是在同样的程度上增加了额外的10 (7)mol / L甲状旁腺激素(甲状旁腺素)或10 (6)mol / L 1、25 dihydroxyvitamin D3(1,25维特D3)。依诺肝素相比,LMWH浓度>或= 100微克/毫升(> = 14.0 anti-Xa单位/毫升)观察最大同位素释放之前被要求。在浓度远高于治疗水平,lmwh刺激45 ca释放只有1.25 + / - 0.01倍。 Heparins with high and low antithrombin III affinities stimulated 45Ca release equally well. Both size and sulfation were found to be major determinants of heparin's ability to promote isotope release. Thus, the ability of defined heparin fragments to stimulate 45Ca release correlated with their molecular weight, and after N-desulfation the ability of heparin to induce isotope release was greatly diminished. Dermatan sulfate had no effect on 45Ca release. We conclude that size and sulfation are major determinants of heparin's ability to promote bone resorption and that the risk of heparin-induced osteoporosis may be reduced by the use of LMWH preparations.