小说carbapenem-hydrolyzing beta-lactamase KPC-1,特株肺炎克雷伯菌。
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Yigit H,昆男,安德森GJ, Domenech-Sanchez,比德尔JW,管家CD,阿尔贝蒂年代,布什K, Tenover FC
小说carbapenem-hydrolyzing beta-lactamase KPC-1,特株肺炎克雷伯菌。
45 Antimicrob代理Chemother。2001年4月;(4):1151 - 61。
- PubMed ID
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11257029 (在PubMed]
- 文摘
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肺炎克雷伯菌分离显示中度到高层imipenem和meropenem抵抗了。这两种药物的麦克风是16 microg /毫升。beta-lactamase活动与imipenem meropenem抑制在克拉维酸的存在。压力也耐extended-spectrum头孢菌素和aztreonam。等电点聚焦研究证明三beta-lactamasesπ为7.2 (SHV-29), 6.7 (KPC-1)和5.4 (TEM-1)。bla (SHV)和bla (TEM)证实了基因特定pcr和DNA序列分析。转换和接合与大肠杆菌的研究表明,beta-lactamaseπ为6.7,KPC-1 (k .肺炎carbapenemase-1),编码在一个大约50 kb nonconjugative质粒。bla (KPC-1)基因,在大肠杆菌克隆,赋予抵抗imipenem, meropenem, extended-spectrum头孢菌素,和aztreonam。小说的氨基酸序列carbapenem-hydrolyzing beta-lactamase, KPC-1,显示,45%身份9.7πcarbapenem-hydrolyzing beta-lactamase, Sme-1,从粘质沙雷氏菌S6。水解的研究表明,纯化KPC-1水解不仅碳青霉烯还青霉素,头孢菌素和monobactams。 KPC-1 had the highest affinity for meropenem. The kinetic studies also revealed that clavulanic acid and tazobactam inhibited KPC-1. An examination of the outer membrane proteins of the parent K. pneumoniae strain demonstrated that the strain does not express detectable levels of OmpK35 and OmpK37, although OmpK36 is present. We concluded that carbapenem resistance in K. pneumoniae strain 1534 is mainly due to production of a novel Bush group 2f, class A, carbapenem-hydrolyzing beta-lactamase, KPC-1, although alterations in porin expression may also play a role.