行效率的药物,靶向细胞内磷酸二酯酶活动:体外研究。

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Muravyov AV, Yakusevich VV Chuchkanov FA, Maimistova AA, Bulaeva SV,扎伊采夫LG

行效率的药物,靶向细胞内磷酸二酯酶活动:体外研究。

中国Hemorheol Microcirc。2007; 36 (4): 327 - 34。

PubMed ID

17502703 (在PubMed

]

文摘

体外研究旨在检查红细胞microrheological参数的变化(红细胞聚集和悬浮液粘度)后细胞孵化一些药物有磷酸二酯酶(PDE)抑制活动(pentoxifylline - 25.0 microg /毫升;drotaverine - 10.0 microg /毫升;长春西汀- 5.0 microg /毫升;罂粟碱- 10.0 microg /毫升;咖啡因- 25.0 microg /毫升;3-isobutyl-1-methylxanthine [IBMX] - 10.0 microg /毫升)。浓度的药物用于体外红细胞microrheology研究是类似于那些可能在静脉输液治疗后病人的血。红细胞被离心分离血液在1400克15分钟和洗3次磷酸缓冲盐(PBS)。红细胞表面的清洗然后在PBS resuspended比容的大约40%。在这些红细胞悬浮液研究会话被分成两个整除和暴露:药物在37摄氏度的15分钟; remaining aliquot (red cell suspension with PBS) was kept at 37 degrees C for 15 min and served as the control. It was found that all of used drugs decreased red cell aggregation and their suspension viscosity significantly. Since IBMX and vinpocetine are the specific inhibitor PDE activity it might be suppose that cellular PDE is molecular target in RBCs for this class of drugs. The obtained data reveals evidence that drugs, acting as PDE inhibitors, might be considered as microrheologically positive remedies.

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药物靶点

药物	目标	类	生物	药理作用	行动
Drotaverine	cAMP-specific 3 ', 5 '环磷酸二酯酶4 a	蛋白质	人类	是的	抑制剂	细节