小说proteoform前列腺特异抗原的识别(SNP-L132I)由多个反应监测在临床样本。
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Vegvari, Sjodin K, Rezeli M,白垩土J, Lilja H, Laurell T, Marko-Varga G
小说proteoform前列腺特异抗原的识别(SNP-L132I)由多个反应监测在临床样本。
摩尔细胞蛋白质组学。2013年10月,12 (10):2761 - 73。doi: 10.1074 / mcp.M113.028365。Epub 2013 7月10。
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23842001 (在PubMed]
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前列腺特异抗原(PSA)是一个完善的肿瘤标记,经常用作生物标记的发展模式和评估新兴定量蛋白质组学技术,部分由于广泛进入商业化分析作为“黄金标准”。We designed a multiple reaction monitoring (MRM) assay to detect PSA proteoforms in clinical samples (n = 72), utilizing the specificity and sensitivity of the method. We report, for the first time, a PSA proteoform coded by SNP-L132I (rs2003783) that was observed in nine samples in both heterozygous (n = 7) and homozygous (n = 2) expression profiles. Other isoforms of PSA, derived from protein databases, were not identified by four unique proteotypic tryptic peptides. We have also utilized our MRM assay for precise quantitative analysis of PSA concentrations in both seminal and blood plasma samples. The analytical performance was evaluated, and close agreement was noted between quantitations based on three selected peptides (LSEPAELTDAVK, IVGGWECEK, and SVILLGR) and a routinely used commercialized immunoassay. Additionally, we disclose that the peptide IVGGWECEK is shared with kallikrein-related peptidase 2 and therefore is not unique for PSA. Thus, we propose the use of another tryptic sequence (SVILLGR) for accurate MRM quantification of PSA in clinical samples.