基因的核苷酸序列b亚基十三人的因素。
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基因的核苷酸序列b亚基十三人的因素。
生物化学,1990年12月25日,29日(51):11195 - 209。
- PubMed ID
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2271707 (在PubMed]
- 文摘
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因素十三世(320000)先生是一个稳定的凝血因子和增强纤维蛋白凝块。它在血液循环由两个亚基组成的四聚物(75000)先生和两个b亚基(80000)先生。b亚基由641个氨基酸,包括10串联重复序列的60个氨基酸被称为GP-I结构、短共识重复序列(SCR),或者寿司域。在目前的研究中,b亚基的人类基因已经从三个不同的孤立在λ噬菌体基因组库。15个独立的噬菌体与插入编码为整个基因分离和映射,以限制印迹,南部和DNA测序。基因被发现28个碱基长度和由12个外显子(I-XII)相隔11干预序列。顺序是由外显子编码我领导,而蛋白质的carbonyl-terminal地区被第十二外显子编码。外显子II-XI每个编码为一个寿司域,这表明通过外显子基因进化的洗牌和重复。12个基因的外显子的大小不等,从64年到222个碱基对,而内含子的大小不等,从87年到9970年核苷酸,由92%的基因。内含子包含四个Alu重复序列,分别在内含子A, E, I, j .五分之一Alu重复出现在侧翼基因的3 '末端。 Two partial KpnI repeats were also found in the introns, including one in intron I and one in intron J. The KpnI repeat in intron J was 89% homologous to a sequence of approximately 2200 nucleotides flanking the gene coding for human beta globin and approximately 3800 nucleotides from the L1 insertion present in the gene for human factor VIII. Intron H also contained an "O" family repeat, while two potential regions for Z-DNA were identified within introns G and J. One nucleotide change was found in the coding region of the gene when its sequence was compared to that of the cDNA. This difference, however, did not result in a change in the amino acid sequence of the protein.