基德人类红细胞血型和尿素运输功能是由相同的蛋白质。
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橄榄B,加尼姆毫克,休伊特M Neau P,战争年代,贝利Cartron JP, P
基德人类红细胞血型和尿素运输功能是由相同的蛋白质。
生物化学杂志。1995年6月30日;270 (26):15607 - 10。
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7797558 (在PubMed]
- 文摘
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该基因编码人类红细胞的尿素运输车(HUT11克隆)最近克隆(橄榄,B。Neau, P。,贝利P。、《m。Rousselet, G。、Cartron j . P。,Ripoche p(1994)生物。269年化学,31649 - 31652)。现在,这个基因被分配到18号染色体q12-q21原位杂交,为基德(Jk)还发现血型轨迹。在耦合transcription-translation化验,HUT11 cDNA导演36-kDa蛋白质的合成由人类免疫沉淀反应anti-Jk3抗体产生的免疫Jk (a - b)捐助者的红细胞缺乏基德抗原。还anti-Jk3抗体免疫沉淀反应蛋白质材料46-60 kDa从红细胞膜,除了那些从Jk (a - b)细胞。N-glycanase消化后被减少到36 kDa 46-60-kDa组件。 A rabbit antibody raised against the predicted NH2-terminal amino-acids of the HUT11 protein reacted on immunoblots with a 46-60-kDa component present in all human erythrocytes except those from Jk(a-b-) individuals. Jk(a-b-) red cells lack the Kidd/urea transport protein and have a selective defect of the urea transport capacity, but a normal water permeability and aquaporin-associated Colton blood group antigens. These findings indicate that the erythrocyte urea transporter is encoded by the Kidd locus and may have implications for the biology of urea transporters and their tissue-specific regulation.