tlyA基因产物的分子特征,Rv1694结核分枝杆菌:非常规溶血素和核糖体核糖核酸甲基转移酶。
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拉赫曼,斯利瓦斯塔瓦党卫军,Sneh艾哈迈德·N Krishnasastry MV
tlyA基因产物的分子特征,Rv1694结核分枝杆菌:非常规溶血素和核糖体核糖核酸甲基转移酶。
学生物化学BMC。2010年9月20;火灾。doi: 10.1186 / 1471-2091-11-35。
- PubMed ID
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20854656 (在PubMed]
- 文摘
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背景:结核分枝杆菌是一种致命的杆菌引起肺结核,疾病负责全球每年数百万人死亡。为了了解其发病机制在人类和帮助控制肺结核、功能众多结核分枝杆菌基因特征。在这项研究中我们报告的双重tlyA基因产物的功能,结核分枝杆菌标注为Rv1694 268氨基酸长基本蛋白质。结果:重组纯化Rv1694蛋白被发现具有体外溶血性活动。它显示浓度和时间兔子和人类红细胞的溶血。多个低聚物的形式(二聚体七聚物)的蛋白质被膜上的细胞溶解红细胞。像传统的寡聚物,众所周知,造孔毒素,Rv1694的寡聚物被发现是耐热和SDS,但容易减少代理像beta-mercaptoethanol已废除的溶血性活动Rv1694指示二硫键的作用。生成的Rv1694新创的体外转录和翻译也表现出明确的溶血确认自我组装和寡聚化这种蛋白质的性质。有限的蛋白水解消化这种蛋白质显示,氨基酸是敏感而在解决方案,但在膜的存在。Rv1694引人注目的特点是它的存在在用共焦显微镜观察大肠杆菌的细胞壁。 The surface expression is consistent with the contact dependent haemolytic ability of E. coli expressing this protein. Also, immune serum specific to this protein inhibits the contact dependent hemolysis. Moreover, Rv1694 protein binds to and forms stable oligomers on the macrophage phagosomal membranes. In addition to all these properties, E. coli expressing Rv1694 was found to be susceptible to the antibiotic capreomycin as its growth was significantly slower than mock vector transformed E. coli. The S30 extract of E. coli expressing the Rv1694 had poor translational activity in presence of capreomycin, further confirming its methylation activity. Finally, incorporation of methyl group of [3H]-S-adenosylmethionine in isolated ribosomes also confirmed its methylation activity. CONCLUSIONS: The Rv1694 has an unusual dual activity. It appears to contain two diverse functions such as haemolytic activity and ribosomal RNA methylation activity. It is possible that the haemolytic activity might be relevant to intra-cellular compartments such as phagosomes rather than cell lysis of erythrocytes and the self-assembly trait may have a potential role after successful entry into macrophages by Mycobacterium tuberculosis.