抗炎和anti-fibrinolytic thrombomodulin阿尔法的影响通过羧肽酶B2在凝血酶的存在。

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Tawara年代,酒井法子T,松崎O

抗炎和anti-fibrinolytic thrombomodulin阿尔法的影响通过羧肽酶B2在凝血酶的存在。

Thromb研究》2016年11月,147:72 - 79。doi: 10.1016 / j.thromres.2016.09.011。Epub 2016年9月17日。

PubMed ID

27693845 (在PubMed

]

文摘

背景:Thrombomodulin (TM)阿尔法,重组人可溶性TM,提高pro-carboxypeptidase B2 (pro-CPB2)凝血酶的激活。激活pro-CPB2 (CPB2)产生抗炎和anti-fibrinolytic活动。因此,TM阿尔法也可能通过CPB2抗炎和anti-fibrinolytic效果。然而,这些影响TM阿尔法尚未阐明。在目前的研究中,我们调查了TM阿尔法对失活的影响补充组件C5a作为抗炎效果和延长血栓溶解的时间作为一个通过体外CPB2 anti-fibrinolytic效应。方法:CPB2活动和组织因素显色测定凝血酶生成做了检查。ca5的失活被C5a c端乳沟的评估,抑制C5a-induced人类嗜中性粒细胞迁移。血栓溶解时间延长是由一个组织类型检查纤溶酶原activator-induced物凝块溶解试验。结果:在人血浆CPB2活动增加了TM阿尔法和凝血酶浓度的方式。TM阿尔法抑制组织因素在人血浆凝血酶生成和增强pro-CPB2激活同时进行。 The mass spectrum of C5a treated with TM alfa, thrombin, and pro-CPB2 was decreased at 156m/z, indicating that TM alfa enhanced the processing of C5a to C-terminal-cleaved C5a, an inactive form of C5a. C5a-induced human neutrophil migration was decreased after C5a treatment with TM alfa, thrombin, and pro-CPB2. TM alfa prolonged the clot lysis time in human plasma, and this effect was completely abolished by addition of a CPB2 inhibitor. CONCLUSIONS: TM alfa exerts anti-inflammatory and anti-fibrinolytic effects through CPB2 in the presence of thrombin in vitro.

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药物

Thrombomodulin阿尔法