硝酸盐还原为亚硝酸盐,一氧化氮在生理条件下由肠道细菌和氨。
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Tiso M, Schechter
硝酸盐还原为亚硝酸盐,一氧化氮在生理条件下由肠道细菌和氨。
《公共科学图书馆•综合》。2015年3月24日,10 (3):e0119712。doi: 10.1371 / journal.pone.0119712。eCollection 2015。
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25803049 (在PubMed]
- 文摘
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生物氮循环包括步进式减少氧化氮盐和氨氧化铵回亚硝酸盐和硝酸盐的植物和细菌。无论是过程已经被认为与哺乳动物生理学;然而近年来,唾液细菌减少硝酸盐亚硝酸盐已经被认为是一个重要的代谢转化。几种肠道细菌也表明硝酸催化还原的能力通过亚硝酸氨在异化的呼吸;然而,这个途径的重要性在人类肠道细菌物种殖民很少研究。我们测量亚硝酸盐,一氧化氮(NO)和氨形成文化的大肠杆菌、乳酸菌和双歧杆菌物种生长在不同硝酸钠浓度和氧气水平。我们发现硝酸5毫米的存在提供了一个经济增长效益和诱导亚硝酸盐和氨代E。杆菌和L。杆菌的细菌生长在氧气浓度与胃肠道中的内容兼容。亚硝酸盐和氨积累在生长介质至少2.5毫米硝酸。时间进程曲线表明,硝酸首先随后转化为亚硝酸盐和氨。 Strains of L.rhamnosus, L.acidophilus and B.longum infantis grown with nitrate produced minor changes in nitrite or ammonia levels in the cultures. However, when supplied with exogenous nitrite, NO gas was readily produced independently of added nitrate. Bacterial production of lactic acid causes medium acidification that in turn generates NO by non-enzymatic nitrite reduction. In contrast, nitrite was converted to NO by E.coli cultures even at neutral pH. We suggest that the bacterial nitrate reduction to ammonia, as well as the related NO formation in the gut, could be an important aspect of the overall mammalian nitrate/nitrite/NO metabolism and is yet another way in which the microbiome links diet and health.
