结构和功能特征的oversulfated硫酸软骨素硫酸/ dermatan脊索的混合链八目鳗类鱼。Neuritogenic为生长因子和神经营养因子和绑定活动。
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伊藤研究员合作Nandini CD,劳斯T M, N, Akiyama-Nambu F, Sugahara K
结构和功能特征的oversulfated硫酸软骨素硫酸/ dermatan脊索的混合链八目鳗类鱼。Neuritogenic为生长因子和神经营养因子和绑定活动。
J生物化学杂志。2004年12月3;279 (49):50799 - 809。Epub 2004年9月22日。
- PubMed ID
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15385557 (在PubMed]
- 文摘
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Oversulfated硫酸软骨素硫酸(CS) / dermatan (DS)混合链纯化脊索的八目鳗类鱼。链(以前叫CS-H八目鳗类鱼)18 kDa的平均分子质量。使用各种chondroitinases成分分析表明各种D-glucuronic酸(GlcUA)和L-iduronic酸(IdoUA)含有二糖不定地更高比例的硫酸GlcUA / IdoUA-GalNAc 4, 6-O-disulfate,露出复杂的c / d混合特性。混合链显示神经突的outgrowth-promoting活动axonic自然,像鱿鱼软骨CS-E的活动和由chondroitinase ABC废除完全消化和部分由chondroitinase AC-I或B消化、暗示的参与GlcUA和IdoUA neuritogenic活动。纯化CS-H BIAcore系统中表现出相互作用与各种heparin-binding蛋白质和神经营养因子(viz.纤维母细胞生长因子2,-10年,-16年和-18年;midkine;pleiotrophin;heparin-binding表皮生长因子生长因子;血管内皮生长因子;脑源性神经营养因子; and glial cell line-derived neurotrophic factor), most of which are expressed in the brain, although fibroblast growth factor-1 and ciliary neurotrophic factor showed no binding. Kinetic analysis revealed high affinity binding of these growth factors and, for the first time, of the neurotrophic factors. Competitive inhibition revealed the involvement of both IdoUA and GlcUA in the binding of these growth factors, suggesting the importance of the hybrid nature of CS-H for the efficient binding of these growth factors. These findings, together with those from the recent analysis of brain CS/DS chains from neonatal mouse and embryonic pig (Bao, X., Nishimura, S., Mikami, T., Yamada, S., Itoh, N., and Sugahara, K. (2004) J. Biol. Chem. 279, 9765-9776), suggest physiological roles of the hybrid chains in the development of the brain.