甲胺磷、敌敌畏、O-methoate在土耳其和二嗪农农药使用与butyrylcholinesterase共价键被质谱分析。
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Tacal O, Lockridge O
甲胺磷、敌敌畏、O-methoate在土耳其和二嗪农农药使用与butyrylcholinesterase共价键被质谱分析。
J: Toxicol。2010年7月,30 (5):469 - 75。doi: 10.1002 / jat.1518。
- PubMed ID
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20229498 (在PubMed]
- 文摘
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有机磷农药在土耳其使用最常见的包括甲胺磷、敌敌畏、O-methoate和二嗪农。这些有毒化学物质或其代谢产物活性部位的共价键butyrylcholinesterase丝氨酸。我们的目标是识别结果的加合物的反应人类butyrylcholinesterase与这些杀虫剂。高纯度人类butyrylcholinesterase治疗20倍摩尔过量的农药。蛋白质变性的沸腾和胰蛋白酶消化。女士和男男光谱HPLC-purified肽被收购MALDI-TOF-TOF 4800质谱仪。发现甲胺磷添加大量的+ 93,符合的甲氧基aminophosphate。一个小数量的加合物的附加质量+ 109也被发现。敌敌畏和O-methoate都让dimethoxyphosphate(+ 108)和monomethoxyphosphate加合物(+ 94)。二嗪农给小说加合物的附加质量+ 152与diethoxythiophosphate一致。 Inhibition of enzyme activity in the presence of diazinon developed slowly (15 h), concomitant with isomerization of diazinon via a thiono-thiolo rearrangement. The isomer of diazinon yielded diethoxyphosphate and monoethoxyphosphate adducts with added masses of +136 and +108. MSMS spectra confirmed that each of the pesticides studied made a covalent bond with serine 198 of butyrylcholinesterase. These results can be used to identify the class of pesticides to which a patient was exposed.