钙和钙调蛋白在胃底部收缩反应。
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Gajdus M, Szadujkis-Szadurski L, Szadujkis-Szadurski R, Glaza我Zalewska M, Szadujkis-Szadurska K, Gurtowska N
钙和钙调蛋白在胃底部收缩反应。
Postepy以及地中海Dosw(在线)。2011年9月5日,65:569 - 73。doi: 10.5604/17322693.958061。
- PubMed ID
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21918260 (在PubMed]
- 文摘
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背景:本研究的主题是测定钙调蛋白和钙的影响在胃底平滑肌收缩。在实验中,作者测试了5 -羟色胺受体激动剂的影响,5 -羟色胺(5 -),引起平滑肌收缩。材料/方法:测试是进行组织分离出老鼠的肚子。雄性Wistar鼠体重220克和360克之间的腹腔内注射麻醉聚氨酯(120毫克/公斤)。胃是解剖,后来胃底部被隔离。组织被放置在培养皿中绝缘器官20毫升的容量,充满了克雷布斯流体。包含在该研究结果平均值+ / - SE。为了确定统计学意义,受体理论使用的原则(Kenakin修改)。结果:根据进行测试,我们可以推断出8 Br cGMP停止反应,胃底平滑肌收缩引起的血清素。使用8 br-cgmp浓度范围的10至300 microM导致减少最大的效果从100%降至46%。 Similar changes were obtained after the use of guanylate cyclase activator (CG) - YC-1. Curves for the contractile activity of serotonin along with an increase of concentration YC-1 are shifted to the right, and the maximum effect of reaction decreases. Increasing concentrations of flunarizine, a calmodulin antagonist, in a concentration-dependent way blocks binding between calcium and calmodulin, and at the same time leads to the shift of concentration-effect curves for serotonin to the right and a decrease of maximum reaction. Increasing concentrations of ODQ, a guanylate cyclase inhibitor lead to statistically significant shift of the curves to the left, decrease of EC(50) value and simultaneous increase of maximum reaction to serotonin. CONCLUSIONS: According to conducted testing, serotonin causes gastric fundus smooth muscle contraction dependent on concentration. Reaction of contraction induced by serotonin is stopped by a calmodulin antagonist, flunarizine. In addition, experiments confirmed participation of cyclical nucleotides in blocking reaction of gastric fundus contraction.