抗病毒活性的抗菌肽,LL-37和衍生肽在季节性流感和大流行性流感病毒。

文章的细节

引用

复制到剪贴板

王Tripathi年代,G,白色M, L,陶本伯杰J,鹿角KL

抗病毒活性的抗菌肽,LL-37和衍生肽在季节性流感和大流行性流感病毒。

《公共科学图书馆•综合》。2015年4月24日,10 (4):e0124706。doi: 10.1371 / journal.pone.0124706。eCollection 2015。

PubMed ID

25909853 (在PubMed

]

文摘

人类LL-37阳离子抗菌肽,最近被证明有抗病毒活性抗甲型流感病毒(IAV)株在体外和体内。在这项研究中,我们比较了抗流感活动LL-37与几个来自LL-37碎片。我们首先测试了肽对季节性H3N2病毒和鼠标改编H1N1病毒株,PR-8。氨基片段,LL-23轻微中和活动对这些菌株。LL-23V9丝氨酸9由缬氨酸取代创建一个连续的疏水表面。LL-23V9已被证明有抗菌活动增加LL-23相比我们现在展示LL-23相比略有增加抗病毒活性。短中央片段,FK-13 KR-12,具有抗菌活性没有抑制IAV。相比之下,长20个氨基酸的中央LL-37碎片(GI-20)类似于LL-37中和活动。没有一个肽抑制病毒红血球凝聚或神经氨酸酶的活动。我们下一个测试活动的肽对H1N1大流行性流感菌株2009(加州/ / 04/09 / H1N1或“Cal09”)。 Unexpectedly, LL-37 had markedly reduced activity against Cal09 using several cell types and assays of antiviral activity. A mutant viral strain containing just the hemagglutinin (HA) of 2009 pandemic H1N1 was inhibited by LL-37, suggested that genes other than the HA are involved in the resistance of pH1N1. In contrast, GI-20 did inhibit Cal09. In conclusion, the central helix of LL-37 incorporated in GI-20 appears to be required for optimal antiviral activity. The finding that GI-20 inhibits Cal09 suggests that it may be possible to engineer derivatives of LL-37 with improved antiviral properties.

beplay体育安全吗DrugBank数据引用了这篇文章

药物

LL-37