Galectin-1研究增生性糖尿病性视网膜病变。

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阿布El-Asrar,艾哈迈德,Allegaert E, Siddiquei MM,阿拉姆K, Gikandi PW, De Hertogh G, Opdenakker G

Galectin-1研究增生性糖尿病性视网膜病变。

Acta角膜切削。2020年2月,98 (1):e1-e12。doi: 10.1111 / aos.14191。Epub 2019年7月18日。

PubMed ID

31318490 (在PubMed

]

文摘

目的:Galectin-1调节内皮细胞功能,促进血管生成。我们调查了假设galectin-1可能参与增殖型糖尿病视网膜病变(PDR)的发病机理。方法:36 PDR的玻璃样本和20个非糖尿病的患者,从13例PDR外层维管组织的膜,大鼠视网膜穆勒和人类视网膜神经胶质细胞研究了酶联免疫吸附试验(ELISA),免疫组织化学和免疫印迹分析。体外血管生成进行了化验和白细胞的粘附galectin-1-stimulated人类视网膜微血管内皮细胞(HRMECs)评估。结果:ELISA分析表明galectin-1和血管内皮生长因子(VEGF)水平明显高于玻璃样本PDR患者比那些来自刻意为比较(p < 0.001)。之间的正相关显著的galectin-1和VEGF水平(r = 0.354;p = 0.022)。在外层膜,免疫组织化学分析表明,galectin-1在血管内皮细胞表达CD31表达,表达alpha-smooth myofibroblasts肌肉肌动蛋白和白细胞表达CD45。galectin-1受体neuropilin-1表达于血管内皮细胞。CD31染色被用作标记评估微血管密度(MVD)。 Significant positive correlation was detected between MVD in epiretinal membranes and the number of blood vessels expressing galectin-1 (r = 0.848; p < 0.001). Western blot analysis demonstrated significant increase of galectin-1 protein in rat retinas after induction of diabetes. ELISA analysis revealed that hydrogen peroxide and cobalt chloride (CoCl2 ) induced upregulation of galectin-1 in Muller cells. Treatment with galectin-1 induced upregulation of VEGF in Muller cells and increased leukocyte adhesion to HRMECs. The galectin-1 inhibitor OTX008 attenuated VEGF-induced HRMECs migration and CoCl2 -induced upregulation of NF-kappaB, galectin-1 and VEGF in Muller cells. CONCLUSIONS: These results suggest that galectin-1is involved in the pathogenesis of PDR.

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药物靶点

药物	目标	类	生物	药理作用	行动
第一项- 008	Galectin-1	蛋白质	人类	是的	抑制剂	细节