有抗性的表达之间的关系因素和药物敏感性在正常人类肾近端小管上皮细胞和肾细胞癌相比。
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仓叶T, Imai Ohkubo-Uraoka N,黑田M, Iidaka Y,田K, Shibasaki T, Ohkawa K
有抗性的表达之间的关系因素和药物敏感性在正常人类肾近端小管上皮细胞和肾细胞癌相比。
肿瘤防治杂志众议员2005年9月,14 (3):601 - 7。
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16077962 (在PubMed]
- 文摘
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表达水平之间的关系的假定的耐药因素和对抗癌药物的敏感性在人类正常肾近端小管上皮细胞(RPTEC)和三种肾细胞癌(RCC)细胞,VMRC-RCW (RCW) OS-RC-2 (OS2) TUHR14TKB (14 tkb),检查。RPTEC展出22 (Pgp)的高表达gamma-glutamyl半胱氨酸合成酶(gammaGCS)和cis-diamminedichloroplatinum (II) (CDDP) resistance-related基因9 (CRR9),低表达的空泡的atp酶(V-ATPase)和没有表达多种药物resistance-associated蛋白1 (MRP1)。14 tkb表现出高的表达gammaGCS CRR9,低表达的Pgp和V-ATPase,没有MRP1的表达式。CRR9 OS2显示高表达,低表达的Pgp, gammaGCS MRP1和没有V-ATPase的表达式。RCW表现出高表达的Pgp, MRP1 CRR9和低表达的gammaGCS V-ATPase。表达水平的阻力因素不同的细胞中。GST活性和GST-pi每个细胞的表达水平相关,和有高水平OS2 RPTEC。的细胞毒性抗癌药物对每个单元格为96 h,对CDDP和阿霉素(DXR) RPTEC和RCW低于在其他细胞。DXR敏感性增强了Pgp抑制剂治疗,维拉帕米,Pgp表达水平的比例,和增加了对CDDP gammaGCS抑制剂,Buthionine sulfoximine,的比例gammaGCS表达水平(对应于谷胱甘肽含量)。虽然在敏感性显著增加CDDP没有观察到治疗的碾压混凝土V-ATPase抑制剂,Bafilomycin, DXR的敏感性Bafilomycin-treated细胞增加约2倍。beplayapp However, no relation between drug sensitivity and V-ATPase expression was observed. The features (such as degree of resistance) varied among the RCC cell lines manifesting many resistance factors or to the contrary, lacking or having lowered resistance factors in comparison with normal cells. Therefore, it is necessary in clinical cancer chemotherapy to determine and measure the level of expression of each resistance factor in respective tumor tissue.