泊咯沙姆188防止缺血再灌注损伤小鼠后腿骨骼模型。
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墨菲广告,麦科马克MC,比沙拉哒,阮JT,兰多夫,沃特金斯MT,李RC,奥斯汀WG Jr
泊咯沙姆188防止缺血再灌注损伤小鼠后腿骨骼模型。
体Reconstr Surg. 2010年6月,125 (6):1651 - 60。doi: 10.1097 / PRS.0b013e3181ccdbef。
- PubMed ID
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20517088 (在PubMed]
- 文摘
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背景:缺血再灌注损伤可以激活通路产生活性氧,可损伤细胞在细胞膜通过创建洞。共聚物表面活性剂如泊咯沙姆188能够密封细胞膜缺陷。作者假定一个单剂泊咯沙姆管理局188年将减少骨胳肌细胞损伤和缺血再灌注损伤后死亡。方法:小鼠接受性常温后腿缺血2小时。动物治疗150 microl泊咯沙姆188或者在三个时间点:右旋糖酐(1)前10分钟缺血;(2)再灌注前10分钟;(3)再灌注后2到4个小时。再灌注24小时后,分析了组织的肌细胞损伤(组织学)和代谢功能障碍(肌腺苷5’三磷酸)。额外的7天组老鼠进行评估死亡率。结果:当188年泊咯沙姆治疗缺血前给予10分钟,伤害降低了84%,从50%右旋糖酐组损伤8%伤害188年泊咯沙姆组(p < 0.001)。 When administered 10 minutes before reperfusion, poloxamer 188 animals demonstrated a 60 percent reduction in injury compared with dextran controls (12 percent versus 29 percent). Treatment at 2 hours, but not at 4 hours, postinjury prevented substantial myocyte injury. Preservation of muscle adenosine 5'-triphosphate paralleled the decrease in myocyte injury in poloxamer 188-treated animals. Poloxamer 188 treatment significantly reduced mortality following injury (10 minutes before, 75 percent versus 25 percent survival, p = 0.0077; 2 hours after, 50 percent versus 8 percent survival, p = 0.032). CONCLUSION: Poloxamer 188 administered to animals decreased myocyte injury, preserved tissue adenosine 5'-triphosphate levels, and improved survival following hind-limb ischemia-reperfusion injury.
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