小说吡哆醛激酶的表达信使rna剪接变体,PKH-T,在人类的睾丸。
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陆方X,周ZM评选,L,阴你,李JM,甄Y,王H,沙JH
小说吡哆醛激酶的表达信使rna剪接变体,PKH-T,在人类的睾丸。
亚洲J Androl。2004年6月,6 (2):83 - 91。
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15154080 (在PubMed]
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目的:识别特异表达的基因在成人和胎儿睾丸和精子。方法:建立人类睾丸互补脱氧核糖核酸微阵列。然后mRNA的成人和胎儿睾丸和精子纯化和探测器是由反转录反应以信使rna为模板。微阵列杂交探针的成人和胎儿睾丸和精子。差异表达基因的核酸序列测定和同源性搜索数据库的基因库。结果:一种新的人类testis-specific基因,PKH-T,确定了组合成人和胎儿睾丸和精子与人类睾丸cDNA探针微阵列。PKH-T互补dna的长度是1 069个基点。互补脱氧核糖核酸序列的克隆是存入基因库(AY303972)和PKH-T也是临时决定GenSymbol (Unigene HS.38041)。PKH-T包含大多数PKH守恒的主题。239氨基酸序列推导出719个基点的开放阅读框(ORF)基因的同源性PKH (U89606)。 PKH-T was specifically and strongly expressed in the testis. Comparison of the differential expressions of PKH and PKH-T in testes of different developmental stages indicated that PKH-T was expressed in the adult testis and spermatozoa, while PKH, in the adult, fetal and aged testes. PKH-T had no expression in the testis of Sertoli cell only and partially spermatogenic arrest patients. CONCLUSION: PKH-T is a gene highly expressed in adult human testis and spermatozoa. It may play an important role in spermatogenesis and could be related to male infertility.