与年龄相关的变化多巴胺D2受体在大鼠心脏和冠状血管。
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Cavallotti C、F Nuti Bruzzone P, Mancone M
与年龄相关的变化多巴胺D2受体在大鼠心脏和冠状血管。
Exp杂志杂志》2002 May-Jun; 29 (5 - 6): 412 - 8。
- PubMed ID
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12010185 (在PubMed]
- 文摘
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1。多巴胺D2受体的分布在大鼠心脏冠状血管和可能的D2受体密度与年龄相关的变化进行了研究。多巴胺的药理特性和解剖位置D2-like受体在大鼠心脏和冠状动脉血管进行了使用组合绑定技术和光学显微镜放射自显影法。2。样品的心脏冠状血管和收获年轻和年老的老鼠。在冰冻切片,多巴胺D2受体是通过选择性标记D2配体,即[3 h] -spiroperidol (spiperone)。抑制进行了研究利用未标记的受体激动剂和/或标记和未标记的定义药理特异性拮抗剂的绑定。生理实验进行演示和许多多巴胺D2受体之间的选择性对抗药物。3所示。[3 h] -Spiroperidol被绑定到部分大鼠心脏和冠状动脉(在某种程度上符合D2-like多巴胺受体)的标签,一个平衡离解常数约为2.4 + / - 0.7 nmol / L和结合位点的最大容量65.8 + / - 4.5 fmol /毫克的蛋白质。 Experiments performed on sections of coronary veins did not allow the evaluation of specific binding. Autoradiography, observed with light microscopy, showed the development of specific silver grains within the whole wall of rat heart and coronary artery. The greater sensitivity to displacement by amisulpride, bromocriptine, domperidone, haloperidol, raclopride and L-sulpiride than to displacement by N-propyl-norapomorphine, quinpirole and clozapine suggests that the binding sites observed in these experiments are likely to belong to the dopamine D2 receptor subtype. 4. Comparing results in young and old rats, we observed numerous significant age-related changes, including a decrease in D2 receptors localized in rat heart and coronary artery wall. These D2 receptors show a specific location, in close relationship with dopaminergic nerve fibres. They decrease with age and their role remains unknown.