β2-adrenergic功能培养大鼠近端小管上皮细胞。
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β2-adrenergic功能培养大鼠近端小管上皮细胞。
杂志。1996年7月,271 (1 Pt 2): F71-7。
- PubMed ID
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8760245 (在PubMed]
- 文摘
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我们进行研究,以确定功能测试版2-adrenoceptors出现在培养大鼠近端小管上皮细胞。确定培养细胞保持极性对钠运输、细胞酸加载。钠酸加载导致刺激运输。暴露acid-loaded细胞碱性细胞外pH值进一步加强运输钠(na 22通量在pH值7.50是68.1 + / - 44%以上pH值7.00,P < 0.05)。培养近端小管基底也表现出86 rb吸收,其中65%是乌本苷敏感。因此培养细胞维持顶端Na / H基底反向转运和Na-K-adenosinetriphosphatase (Na-K-ATPase)。奥西那林(10 (6)M),选择性β2受体激动剂,刺激Na-K-ATPase活动36 + / - 6%以上控制(P < 0.05)。刺激效应被ici - 118551,选择性β2-antagonist。确定metaproterenol-dependent增加Na-K-ATPase依赖顶端钠条目,顶端条目被dimethylamiloride或最大化与莫能菌素。dimethylamiloride和莫能菌素预防异丙喘宁Na-K-ATPase激活。 Metaproterenol-mediated increases in Na-K-ATPase activity were associated with increases in sodium transport (27 +/- 10% above control, P < 0.05), which was prevented by dimethylamiloride. In contrast to isoproterenol, metaproterenol did not stimulate cAMP production. In summary, we have shown that functional beta 2-adrenoceptors are present on cultured rat proximal tubules. beta 2-Adrenoceptor activation results in increases in Na-K-ATPase and Na transport as a consequence of increased apical sodium entry.