基因组的组织和表达人类b-adrenergicα1受体。
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Ramarao CS, denk JM,佩雷斯DM Gaivin RJ,里克•RP,格雷厄姆RM
基因组的组织和表达人类b-adrenergicα1受体。
J生物化学杂志。1992年10月25日,267 (30):21936 - 45。
- PubMed ID
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1328250 (在PubMed]
- 文摘
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α1-Adrenergic受体(ARs)的鸟嘌呤nucleotide-binding protein-coupled受体超家族。所描述的所有基因ARs intronless迄今为止。我们报告的克隆和基因的核苷酸序列为人类α1 b-ar。它由两个外显子和一个大型基因内区中断的至少20个碱基编码区末端的假定的第六个跨膜域。推导的氨基酸序列编码的受体有高度同源性克隆的仓鼠,鼠,狗α1 b-ars。描述编码的蛋白,融合基因构造,外显子拼接在一起1和外显子2表达在COS-1细胞是暂时性的。转染基因融合产品导致了生产的α1 b-ar配体结合特色的仓鼠α1 b cDNA表达。证据表明人类α1 b-ar孤立的确是转录是基因的发现类似的大小(2.8千碱基)记录在人类心脏和其他组织通过北部污点分析当外显子1或外显子2是用作探针。此外,使用引物设计跨外显子1 /外显子2边界,单链DNA聚合酶链反应产品产生准备从人类心脏mRNA的确切大小和核苷酸序列预测一个外显子1的成绩单是外显子2的拼接。5 '侧翼地区(924碱基对(bp))的外显子1包含TATA盒和CAAT框,但都不是在GC含量高(70%)和包含几个Sp1结合位点(GC盒),符合看家基因的启动子描述。 The 5'-untranslated region also contains a putative cyclic AMP response element. Primer extension studies and RNase protection assays suggested that there are several potential transcription start sites in most tissues with a predominant site located 173 bp upstream from the translation start site. The 3'-flanking region contains a putative polyadenylation signal (ATTAAA) 492 bp downstream from the stop codon. The genomic organization of the human alpha 1B-AR with a single large intron interrupting its coding region differs from those of other ARs as well as muscarinic and 5-hydroxy-tryptamine receptors, which are intronless. The location of the intron in the human alpha 1B-AR gene is also unique among those members of the G-protein-coupled receptor family that do possess introns.(ABSTRACT TRUNCATED AT 400 WORDS)