α(2)巨球蛋白,PSA结合蛋白,是人类前列腺间质中表达。
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巴斯克斯林VK、王SY Boetticher数控,DV, Saboorian H,麦康奈尔JD, Roehrborn CG
α(2)巨球蛋白,PSA结合蛋白,是人类前列腺间质中表达。
前列腺癌。2005年5月15日,63 (3):299 - 308。
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15611997 (在PubMed]
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背景:良性前列腺增生(BPH)的特点是作为基质的过程。基质平滑肌(SM)可能改变其表型在BPH的进展。我们已经识别出可能差异表达基因转录本在BPH使用微分显示方法。孤立的片段中,α(2)巨球蛋白(α(2)- m)是最有趣的。α(2)- m是一个结合蛋白的各种蛋白酶,包括前列腺特异抗原(PSA)。它还在分子捕获和目标中扮演的角色。在这项研究中,我们为α(2)- m表达在人类前列腺癌。方法:微分显示被用来确定和隔离之间的差异表达记录正常前列腺癌和良性前列腺增生组织。rt - pcr、免疫印迹、原位杂交和免疫组织化学是用来确认和描述α(2)- m表达在前列腺癌。结果:实时rt - pcr结果显示,增加3.2倍α(2)- m mRNA表达在前列腺肥大与正常的前列腺组织。 A 1.9-fold increase at protein level was also observed. In situ hybridization and immunohistochemistry showed that alpha(2)-M expression is primarily localized to the stromal compartment. Cultured primary stroma cells maintained alpha(2)-M expression, while prostate epithelial cells had a significantly lower level of alpha(2)-M expression. Furthermore, stromal cells in culture produce and secrete alpha(2)-M in the medium. CONCLUSIONS: We identified alpha(2)-M expression in the human prostate. An increased alpha(2)-M expression appears to be associated with BPH. Considering the unique features of its protein binding and targeting properties, alpha(2)-M expressed in the prostate may play an important role in regulating benign and malignant prostatic growth.