结构分析的序列编码的一种诱导26-kDa蛋白质在人类成纤维细胞。
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Haegeman G、内容J Volckaert G, Derynck R, Tavernier J,菲尔W
结构分析的序列编码的一种诱导26-kDa蛋白质在人类成纤维细胞。
欧元。1986年9月15日,159 (3):625 - 32。
- PubMed ID
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3758081 (在PubMed]
- 文摘
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当人类成纤维细胞被刺激保利(I) X保利(C)在环己酰亚胺的生产β干扰素(IFN-beta) 26-kDa蛋白可以通过抗血清免疫沉淀反应提高了对部分净化人类IFN-beta[内容,J。,智慧,L。Pierard D。Derynck, R。,大肠和菲尔德Clercq w . Proc。《Sci》(1982)。美国79年,2768 - 2772]。在我们的手中这26-kDa蛋白质没有抗病毒活性。其他调查人员,然而,报道了在相同条件下的第二种干扰素,所谓的beta 2物种[Weissenbach J。Chernajovsky, Y。Zeevi, M。舒尔曼,L。Soreq, H。近红外光谱,U。瓦拉赫,D。Perricaudet, M。Tiollais, p &陶醉,m . Proc。《Sci》(1980)。 USA 77, 7152-7156] of which the mRNA structure and protein characteristics strongly suggests identity with the 26-kDa product. In this paper we describe the nucleotide sequence of the 26-kDa cDNA and part of the corresponding genomic clone. The cDNA clones were isolated from a library made with mRNA from induced human fibroblasts. As, however, the information thus obtained was still incomplete, genomic clones were isolated from a total human DNA library. In this way, the entire region coding for the 26-kDa protein was established, as well as the neighbouring sequences including the inducible promoter area. From the deduced polypeptide sequence a number of characteristics of the 26-kDa protein can be explained. It turns out that the 26-kDa protein gene and the so-called 'IFN-beta 2' gene are identical. However, extensive homology searches indicate that the 26-kDa protein does not show statistically significant sequence homology with any known interferon species. Hence, the question of whether the 26-kDa product represents a novel IFN species remains open.