xanthopsins:一个新的家庭eubacterial探讨蓝光光感受器。
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西方Kort R,霍夫WD,范M,克朗AR, Hoffer SM, Vlieg KH Crielaand W, Van Beeumen JJ, Hellingwerf KJ
xanthopsins:一个新的家庭eubacterial探讨蓝光光感受器。
EMBO j . 1996年7月1;15 (13):3209 - 18。
- PubMed ID
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8670821 (在PubMed]
- 文摘
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黄色光活化的蛋白(PYP)是一个光感受器,已经从三个孤立的嗜盐的光养紫色细菌。PYP的外硫红螺菌属halophila BN9626是唯一的序列已经被报道在DNA水平。在这里,我们描述了克隆和测序的基因编码的pyp E。halophila SL-1(应变)和Rhodospirillum salexigens。后者蛋白质包含,如E。halophila PYP,发色团反式p-coumaric酸,我们显示在高效毛细管区带电泳。此外,我们目前的证据的存在一个基因编码一个PYP同族体在Rhodobacter sphaeroides,第一个基因良好的细菌这种感光已被确认。pyp的ORF下游基因E。halophila编码一种酶,它参与提出了PYP的发色团的生物合成。pyp基因从E。halophila用于异种的超表达在大肠杆菌和R。sphaeroides, aimed at the development of a holoPYP overexpression system (an intact PYP, containing the p-coumaric acid chromophore and displaying the 446 nm absorbance band). In both organisms the protein could be detected immunologically, but its yellow color was not observed. Molecular genetic construction of a histidine-tagged version of PYP led to its 2500-fold overproduction in E.coli and simplified purification of the heterologously produced apoprotein. HoloPYP could be reconstituted by the addition of p-coumaric anhydride to the histidine-tagged apoPYP (PYP lacking its chromophore). We propose to call the family of photoactive yellow proteins the xanthopsins, in analogy with the rhodopsins.