白色念珠菌的大规模检查参与组成分泌腺的光谱分析。
文章的细节
-
引用
复制到剪贴板 -
芦粟AG) Heilmann CJ,德克霍奇金淋巴瘤,Brul年代,de Koster CG Klis调频
白色念珠菌的大规模检查参与组成分泌腺的光谱分析。
酵母。2010年8月,27 (8):661 - 72。doi: 10.1002 / yea.1775。
- PubMed ID
-
20641015 (在PubMed]
- 文摘
-
白色念珠菌致病性真菌分泌相当数量的水解酶和其他蛋白质。检查参与组成分泌腺白念珠菌的深入研究可以为诊断标记和疫苗的研制提供新的候选人。我们比较不同生长条件不同的pH值、温度和菌丝的诱导物的存在N-acetylglucosamine。的多肽内容媒体增长0.1总生物量的-0.2%。使用LC-tandem质谱分析,我们确定了44分泌蛋白质,跨膜蛋白Msb2 6分泌pathway-associated蛋白质和28日预测胞质蛋白。许多分泌蛋白质wall-related,表明他们的存在在生长介质至少部分是由于意外释放从墙上。Als3、Csa2 Rbt4, Sap4 Sap6浓缩在菌丝的媒介文化;Bgl2、Cht3 Dag7、Eng1 Pir1, Rbe1, Scw11, Sim1 / Sun42 Xog1 Ywp1在酵母细胞的培养基;和Plb4.5 pH值4中。七个蛋白(Cht3、Mp65 Orf19.5063 / Coi1 Scw11, Sim1, Sun41和Tos1)一直存在在所有条件下测试。 These observations indicate that C. albicans tightly regulates its secretome. Mp65, Sun41, and Tos1 were each predicted to contain at least one highly immunogenic peptide. In total, we identified 29 highly immunogenic peptides originating from 18 proteins, including two members of the family of secreted aspartyl proteases. Fifty-six peptides were identified as proteotypic and will be useful for quantification purposes. In summary, the number of identified secretory proteins in the growth medium has been substantially extended, and growth conditions strongly affect the composition of the secretome.