完整的序列IncP-9 TOL从假单胞菌质粒pWW0 putida。
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大,Lambertsen L,威廉姆斯PA,托马斯厘米
完整的序列IncP-9 TOL从假单胞菌质粒pWW0 putida。
环境Microbiol。2002年12月;4 (12):856 - 71。
- PubMed ID
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12534468 (在PubMed]
- 文摘
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托尔质粒pWW0 (117 kb)是最好的研究降解质粒和IncP-9质粒不相容的原型从假单胞菌群。其携带降解甲苯和二甲苯(xyl)基因在异化的转座子Tn4651 Tn4653。分析完成pWW0核苷酸序列显示148假定的开放阅读框架。其中,77显示相似序列发表在可用的数据库预测功能:质粒复制,稳定的维护和转移;表型决定因素;基因调控和表达;和换位。所有可识别的换位功能躺在70 kb的边界转座子Tn4653,留下一个46 kb部门包含所有IncP-9核心功能。复制子稳定继承地区非常类似于mini-replicon IncP-9抗生素耐药性质粒量子化学,和他们的代表蛋白质形成一个小说群起始蛋白质。pWW0转移函数存在两个街区编码假定的DNA处理和接合对形成基因,与组织和IncW质粒序列相似性。 In addition to the known Tn4651 and IS1246 elements, two additional transposable elements were identified as well as several putative transposition functions, which are probably genetic remnants from previous transposition events. Genes likely to be responsible for known resistance to ultraviolet light and free radicals were identified. Other putative phenotypic functions identified included resistance to mercury and other metal ions, as well as to quaternary ammonium compounds. The complexity and size of pWW0 is largely the result of the mosaic organization of the transposable elements that it carries, rather than the backbone functions of IncP-9 plasmids.