比较myristoyl-CoA:蛋白质N-myristoyltransferases从三种病原真菌:新型隐球菌荚膜组织胞浆菌、白色念珠菌。
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洛奇JK,约翰逊RL,温伯格RA,戈登霁
比较myristoyl-CoA:蛋白质N-myristoyltransferases从三种病原真菌:新型隐球菌荚膜组织胞浆菌、白色念珠菌。
生物化学杂志。1994年1月28日,269 (4):2996 - 3009。
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8300631 (在PubMed]
- 文摘
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Myristoyl-CoA:蛋白质N-myristoyltransferase十四烷酸(Nmt)转移从农委会氨基g残留细胞蛋白质的有序反应机理,首先涉及到绑定的Myristoyl-CoA酶蛋白。该基因编码酿酒酵母Nmt1p (NMT1)对营养生长至关重要。白色念珠菌、新型隐球菌var. neoformans,荚膜组织胞浆菌的主要原因是系统性真菌感染人类免疫功能不全的。代谢标记的研究表明,他们合成一组小的细胞指数增长期间N-myristoylproteins富媒体,其中最著名的co-migrate有两个基本功能可互换的酿酒酵母N-myristoylproteins, ADP核糖基化因子- 1 (Arf1p)和Arf2p。NMT和新生隐球菌和h ARF基因已经被从capsulatum使用聚合酶链反应。他们是单拷贝基因,打断了多个内含子。c . neoformans和h . capsulatum nmt有大约50%的氨基酸序列的身份与同源酵母,白念珠菌、新生隐球菌和h和智人N-myristoyltransferases,而capsulatum Arf大约有80%是相同的与白念珠菌Arf和酿酒酵母Arf1p Arf2p。新生隐球菌和功能性的研究白念珠菌nmt在大肠杆菌进行显示,(我)高效酰化酿酒酵母Arf2p;新生隐球菌Arf (ii)是一个新生隐球菌Nmt的基质;和(iii)替换一个Asp通用电气位于5 C末端的残留这两个酶导致催化效率明显降低与温度有关的,就像它与酿酒酵母和智人核磁测井仪。 Wild type C. neoformans, C. albicans, and H. sapiens NMTs can fully complement the lethal phenotype of a S. cerevisiae nmt1 null allele at 24 and 37 degrees C when the GAL1-10 promoter controlling their expression is induced by galactose. Only the C. albicans enzyme is able to do so when the promoter is repressed with glucose. This complementation profile likely arises, at least in part, from differences in the protein substrate specificities of the orthologous Nmts. A Gly-->Asp mutation in S. cerevisiae, C. neoformans, C. albicans, and H. sapiens Nmts produces temperature-sensitive growth arrest in isogenic S. cerevisiae strains with a nmt1 null allele. Growth of strains producing the mutant C. albicans or H. sapiens, but not the C. neoformans, enzyme can be rescued by myristate at the non-permissive temperature (37 degrees C) even in the presence of cerulenin, an inhibitor of fatty acid synthetase.(ABSTRACT TRUNCATED AT 400 WORDS)