克隆和表征N-acetylglucosamine操纵子的大肠杆菌。
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仙女公斤,戈尔迪H, Waygood EB
克隆和表征N-acetylglucosamine操纵子的大肠杆菌。
生物化学细胞杂志。1990年1月,68 (1):123 - 37。
- PubMed ID
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2190615 (在PubMed]
- 文摘
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三个酶所需N-acetylglucosamine(唠叨)利用大肠杆菌:酶IInag(基因内奇),N-acetylglucosamine-6-phosphate脱乙酰酶(基因那加人),和glucosamine-6-phosphate异构酶(基因nagB)。这三个基因附近16分钟在大肠杆菌染色体上。大肠杆菌、KPN9无法利用N-acetylglucosamine,被用来屏幕大肠杆菌的基因组文库补充重组大肠杆菌素E1 N-acetylglucosamine质粒,允许增长。质粒pLC5-21被发现包含所有三个已知基因唠叨5.7千碱基(5.7 kb)的DNA片段。这些唠叨基因被确定的产品互补突变的大肠杆菌菌株椒,nagB和内奇。5.7 kb的基因产物片段被确定(35 s] methionine-labelled大型细胞和放射自显影法钠十二烷基sulphate-polyacrylamide电泳凝胶。基因产物有以下相对质量(夫人:内奇,62000年;那加人,45000;nagB, 29000年。此外,先生44000年发现的另一个产品。 The genes have been sequenced to reveal an additional open reading frame (nagC), a putative catabolite activator protein binding site that may control nagB and nagE, putative rho-independent terminator sites for nagB and nagE, and sequence homologies for RNA polymerase binding sites preceding each of the open reading frames, except for nagA. The calculated molecular weight (MWs) of the gene products derived from the sequence are as follows: nagA, 40,954; nagB, 29,657; nagC, 44,664; nagE, 68,356. No role is known for nagC, although a number of regulatory roles appear to be plausible. No obvious transcriptional termination site distal to nagC was found and another open reading frame begins after nagC. This gene, nagD, was isolated separately from pLC5-21, and the sequence revealed a protein with a calculated MW of 27,181. The nagD gene is followed by repetitive extragenic palindromic sequences. The nag genes appear to be organized in an operon: nagD nagC nagA nagB nagE.