大肠杆菌的支链氨基酸转氨酶:生产过剩和属性。
文章的细节
-
引用
复制到剪贴板 -
井上K, Kuramitsu年代,阿基K,渡边Y,高木涉T, Nishigai M, Ikai, Kagamiyama H
大肠杆菌的支链氨基酸转氨酶:生产过剩和属性。
1988年11月,104 (5):777 - 84。
- PubMed ID
-
3069843 (在PubMed]
- 文摘
-
ilvE大肠杆菌的基因插入到下游地区tac的催化剂。结果,支链氨基酸转氨酶在大肠杆菌W3110过度繁殖了一棵橡树。beplayapp过度繁殖的转氨酶是同质性从细胞中提取纯化40倍。beplayapp化学和物理化学分析证实了这是一个ilvE基因的产物。酶存在于一个六聚体亚基分子量为34000;酶的双三聚物模型假定由前化学交联实验(Lee-Peng F.-C。j . bacteriol et al。(1979)。139年,339 - 345)是支持的电子显微图。圆二向色(CD)光谱的支链氨基酸转氨酶有双重否定最大值在210和220海里。α螺旋的内容从CD谱估计约40%在200到250纳米。beplayapp The absorption spectrum of the enzyme showed two peaks at 330 and 410 nm. There was no pH-dependent spectral shift. The CD spectrum of the coenzyme, pyridoxal 5'-phosphate, had negative peaks at 330 and 410 nm. These spectral properties of branched-chain amino acid aminotransferase were quite different from those of E. coli aspartate aminotransferase. Each subunit bound approximately 1 mol of pyridoxal 5'-phosphate. A lysyl residue, which forms a Schiff base with the aldehyde group of the pyridoxal 5'-phosphate, was identified in the primary structure of the enzyme.