降低窦房结营内容扮演了一个角色在兔子的产后心率减慢。
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杨ZF,太阳Y,李CZ,王HW,王XJ,郑YQ,刘K,刘
降低窦房结营内容扮演了一个角色在兔子的产后心率减慢。
中国Exp杂志杂志》2006年8月,33 (8):757 - 62。
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16895552 (在PubMed]
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1。在开发过程中降低心率是副交感神经系统成熟的结果,压低了起搏器目前(如果)乙酰胆碱(ACh)。然而,直接影响ACh如果已经排除了和其他二级信使的参与,如营地,在先前的研究中得到证实。因此,我们假设减少基底在窦房结营生产(SA)节点细胞可能有助于减缓心率出生后。2。心电图和心率变异性(HRV)记录和测量体内和体外(包括Langendorff制剂)对兔子2岁,4,6,8 - 12周。窦房结动作电位(AP)记录和穿孔膜片箝分析被用来研究自发的去极化率,如果电流起搏器。在SA浓度节点组织测定等。腺苷酸环化酶的相对表达(ADCY1 5)和磷酸二酯酶(PDE1A 4和8)被即时逆转录-聚合酶链反应量化。3所示。 Significantly reduced heart rate, but unchanged HRV, was observed in perfused hearts in the older age groups, accompanied with a slowed phase 4 spontaneous depolarization rate (90.5 +/- 4.7 vs 49.6 +/- 2.6 mV/s for 2 week vs 4 week hearts, respectively; n = 5; P < 0.05), a negative shift of the If threshold potential (-45.5 +/- 3.0 vs -51.1 +/- 6.0 mV for 2 week vs 4 week hearts, respectively; n = 9; P < 0.05) and decreasing basal levels of SA nodal cAMP (0.31 +/- 0.05 vs 0.025 +/- 0.002 micromol/L for 2 week vs 4 week hearts, respectively; n = 6; P < 0.05). Gene expression levels of PDE1A, 4A and 8A were increased in the 12 week group compared with the 2 week group 1.5-, 2- and 1.8-fold, respectively (P < 0.05), with little change in ADCY1 and 5. 4. These data suggest that, in addition to autonomic innervation, slowing of heart rate during postnatal maturation can be attributed to a negative shift of the If activation caused by diminished baseline cAMP content in SA nodal cells.