脯氨酸的生物合成在铜绿假单胞菌。gamma-glutamyl磷酸还原酶和1-pyrroline-5-carboxylate还原酶的性质。

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克里希纳房车,Beilstein P,莱辛格T

脯氨酸的生物合成在铜绿假单胞菌。gamma-glutamyl磷酸还原酶和1-pyrroline-5-carboxylate还原酶的性质。

j . 1979 7月1;181 (1):223 - 30。

PubMed ID

114173年(在PubMed

]

文摘

gamma-Glutamyl磷酸还原酶,第二个脯氨酸生物合成的酶,催化作用形成l-glutamic酸5-semialdehyde gamma-Glutamyl磷酸与NAD (P) H作为辅助因子。从原油中提取纯化150倍的铜绿假单胞菌PAO 1 deae纤维素色谱和羟磷灰石吸附色谱法。化验时部分纯化制备,生物合成的扭转方向,利用l-1-pyrroline-5-carboxylic酸为底物,减少NAD (P) (+)。明显的K (m)的值是:NAD(+), 0.36毫米;辅酶ii(+), 0.31毫米;l-1-pyrroline-5-carboxylic酸,4毫米与辅酶ii(+)和8毫米NAD (+);P (i), 28毫米。(3)- Phosphonoacetylamido -l-alanine, gamma-glutamyl磷酸盐的结构类似物,抑制这种酶竞争性(K (i) = 7毫米)。1-Pyrroline-5-carboxylate还原酶(EC 1.5.1.2),第三个酶脯氨酸的生物合成,纯化五六折了(NH(4))(2)(4)分馏,交联葡聚糖凝胶g - 150过滤和deae纤维素色谱法。它减少了l-1-pyrroline-5-carboxylate NAD (P) H作为l-proline代数余子式。 NADH (K(m)=0.05mm) was a better substrate than NADPH (K(m)=0.02mm). The apparent K(m) values for l-1-pyrroline-5-carboxylate were 0.12mm with NADPH and 0.09mm with NADH. The 3-acetylpyridine analogue of NAD(+) at 2mm caused 95% inhibition of the enzyme, which was also inhibited by thio-NAD(P)(+), heavy-metal ions and thiol-blocking reagents. In cells of strain PAO 1 grown on a proline-medium the activity of gamma-glutamyl kinase and gamma-glutamyl phosphate reductase was about 40% lower than in cells grown on a glutamate medium. No repressive effect of proline on 1-pyrroline-5-carboxylate reductase was observed.

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药物靶点

药物	目标	类	生物	药理作用	行动
谷氨酸	Delta-1-pyrroline-5-carboxylate合酶	蛋白质	人类	未知的	不可用	细节