组我mGlu受体刺激抑制activation-induced人类T淋巴细胞的细胞死亡。

文章的细节

引用

复制到剪贴板

Chiocchetti, Miglio G, Mesturini R, Varsaldi F, Mocellin M, Orilieri E, Dianzani C, Fantozzi R, Dianzani U, Lombardi G

组我mGlu受体刺激抑制activation-induced人类T淋巴细胞的细胞死亡。

Br J杂志。2006年7月,148 (6):760 - 8。Epub 2006 6月5。

PubMed ID

16751798 (在PubMed

]

文摘

1。在activation-induced L-glutamate细胞死亡的影响(上市)的人工激活(1 microg毫升(1)植物凝集素+ 2 U ml(1)白介素2;8天)T淋巴细胞进行了研究通过测量anti-CD3单克隆抗体(10 microg毫升(1);18 h)全身的细胞凋亡(膜联蛋白V和propidium碘染色)。2。L-Glutamate (1 x 10 (8) 1 x 10(4)米)显著(P <或= 0.01)抑制浓度的方式上市(EC50 = 6.3 x 10(8)米;最大抑制54.8 + / - -6.3% 1 x 10 (6) M)。3。L-glutamate的抑制作用是药物的特点是由集团我mGlu受体,因为mGlu受体受体激动剂复制这种效果。EC50值:3.2 x 10 (7) M (1 s, 3 r) acpd学生;4.5 x 10 (8) M quisqualate; 1.0 x 10(-6) M for (S)-3,5-DHPG; 2.0 x 10(-5) M for CHPG. 4. Group I mGlu receptor antagonists inhibited the effects of quisqualate 1.0 x 10(-6) M. The IC50 values calculated were: 8.7 x 10(-5), 4.3 x 10(-6) and 6.3 x 10(-7) M for AIDA, LY 367385 and MPEP, respectively. 5. L-Glutamate (1 x 10(-6) M; 18 h) significantly (P < or = 0.05) inhibited FasL expression (40.8+/-11.3%) (cytofluorimetric analysis), whereas it did not affect Fas signalling. 6. Expression of both mGlu1 and mGlu5 receptor mRNA by T lymphocytes and T-cell lines, as demonstrated by reverse transcriptase-PCR analysis, suggests that L-glutamate-mediated inhibition of AICD was exerted on T cells. 7. These data depict a novel role for L-glutamate in the regulation of the immune response through group I mGlu receptor-mediated mechanisms.

beplay体育安全吗DrugBank数据引用了这篇文章

药物靶点

药物	目标	类	生物	药理作用	行动
谷氨酸	Metabotropic谷氨酸受体1	蛋白质	人类	未知的	不可用	细节