比较在不同的乳酸杆菌蛋白水解活动。
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佐佐木M,博斯曼BW, Tan PS
比较在不同的乳酸杆菌蛋白水解活动。
J乳制品研究》1995年11月,62 (4):601 - 10。
- PubMed ID
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8568030 (在PubMed]
- 文摘
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总共有169乳酸菌菌株从12种(磅嗜酸的,磅短,磅buchneri,磅干酪乳杆菌,磅delbrueckii无性系种群。发酵剂,保加利亚磅delbrueckii无性系种群。delbrueckii,磅delbrueckii无性系种群。lactis,磅酵母、磅helveticus磅paracasei无性系种群。paracasei、磅杆菌和磅喂食),隔绝生牛奶和各种奶制品,和9 Lactococcus lactis菌株与五显色底物肽酶活动的评估和胰蛋白酶的消化酪蛋白。在每个物种,提取游离的肽酶活性的菌株不同。此外,乳酸菌种类和Lc之间的差异观察。lactis。磅helveticus最高的水解活动对所有基质,氨肽酶的强大,X-prolyl-dipeptidyl氨基肽酶和脯氨酸iminopeptidases。磅delbrueckii无性系种群。 bulgaricus possessed high hydrolysing activities towards substrates containing proline, alanyl-prolyl-p-nitroanilide and prolyl-p-nitroanilide. On the other hand, Lb. fermentum and Lb. brevis could be considered as weakly proteolytic species. A more detailed study with highly proteolytic Lactobacillus strains indicated that at least three different proteinases or endopeptidases were present. Compared with Lc. lactis, the Lactobacillus strains had a much lower hydrolytic action on glutamyl-glutamic acid, suggesting that glutamyl aminopeptidase was absent in lactobacilli.