Uncovering global SUMOylation signaling networks in a site-specific manner.
Article Details
-
Citation
Copy to clipboard -
Hendriks IA, D'Souza RC, Yang B, Verlaan-de Vries M, Mann M, Vertegaal AC
Uncovering global SUMOylation signaling networks in a site-specific manner.
Nat Struct Mol Biol. 2014 Oct;21(10):927-36. doi: 10.1038/nsmb.2890. Epub 2014 Sep 14.
- PubMed ID
-
25218447 [View in PubMed]
- Abstract
-
SUMOylation is a reversible post-translational modification essential for genome stability. Using high-resolution MS, we have studied global SUMOylation in human cells in a site-specific manner, identifying a total of >4,300 SUMOylation sites in >1,600 proteins. To our knowledge, this is the first time that >1,000 SUMOylation sites have been identified under standard growth conditions. We quantitatively studied SUMOylation dynamics in response to SUMO protease inhibition, proteasome inhibition and heat shock. Many SUMOylated lysines have previously been reported to be ubiquitinated, acetylated or methylated, thus indicating cross-talk between SUMO and other post-translational modifications. We identified 70 phosphorylation and four acetylation events in proximity to SUMOylation sites, and we provide evidence for acetylation-dependent SUMOylation of endogenous histone H3. SUMOylation regulates target proteins involved in all nuclear processes including transcription, DNA repair, chromatin remodeling, precursor-mRNA splicing and ribosome assembly.
DrugBank Data that Cites this Article
- Polypeptides
-
Name UniProt ID B-cell receptor CD22 P20273 Details DNA topoisomerase 2-alpha P11388 Details Calpastatin P20810 Details DNA topoisomerase 1 P11387 Details 78 kDa glucose-regulated protein P11021 Details Proto-oncogene c-Fos P01100 Details Poly [ADP-ribose] polymerase 1 P09874 Details Keratin, type II cytoskeletal 8 P05787 Details Histone deacetylase 1 Q13547 Details Histone deacetylase 2 Q92769 Details DNA topoisomerase 2-beta Q02880 Details Keratin, type II cytoskeletal 7 P08729 Details Nuclear receptor coactivator 2 Q15596 Details Nuclear receptor-interacting protein 1 P48552 Details Heat shock cognate 71 kDa protein P11142 Details 60S ribosomal protein L26-like 1 Q9UNX3 Details Calcium/calmodulin-dependent protein kinase type 1D Q8IU85 Details Jun dimerization protein 2 Q8WYK2 Details Cyclic AMP-dependent transcription factor ATF-4 P18848 Details CCAAT/enhancer-binding protein beta P17676 Details Protein PML P29590 Details Nucleolar and coiled-body phosphoprotein 1 Q14978 Details Heterogeneous nuclear ribonucleoproteins A2/B1 P22626 Details Heterogeneous nuclear ribonucleoprotein L P14866 Details Splicing factor, proline- and glutamine-rich P23246 Details Stress-induced-phosphoprotein 1 P31948 Details Hepatoma-derived growth factor P51858 Details Ran-specific GTPase-activating protein P43487 Details Heterogeneous nuclear ribonucleoprotein K P61978 Details SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1 Q969G3 Details Chromobox protein homolog 5 P45973 Details Filamin-A P21333 Details Nucleophosmin P06748 Details 60S ribosomal protein L14 P50914 Details Probable ATP-dependent RNA helicase DDX5 P17844 Details Splicing factor 3B subunit 1 O75533 Details Serine/threonine-protein kinase PRP4 homolog Q13523 Details Proliferation marker protein Ki-67 P46013 Details