腺苷A1受体信使rna表达和系统性的影响茶碱管理呼吸功能C2一半切除后4个月。
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Nantwi KD、手工GJ Goshgarian HG
腺苷A1受体信使rna表达和系统性的影响茶碱管理呼吸功能C2一半切除后4个月。
J脊髓。2003年冬季,26 (4):364 - 71。
- PubMed ID
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14992338 (在PubMed]
- 文摘
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背景:从我们实验室之前的研究已经表明,在动物模型的急性颈脊髓损伤(SCI)、呼吸功能可以恢复茶碱。我们还表明,呼吸恢复后发生自发postinjury延长生存期当偏侧膈麻痹的身体的同侧的上颈椎脊髓半切术(C2)。茶碱介导中枢神经系统功能恢复的腺苷A1受体拮抗;然而,目前尚不清楚腺苷酸受体后改变长期postinjury期和茶碱能否进一步增强自发恢复呼吸功能。目的:评估假定的系统性影响茶碱管理进一步加强自发呼吸肌肉恢复4个月在老鼠和C2一半切除后确定是否腺苷A1受体信使rna表达改变在这些动物。方法:在膈神经电生理学的评估呼吸活动是在C2把老鼠进行的半切术在标准条件下后4个月。之后,老鼠立即被杀,颈脊髓准备腺苷A1受体信使rna表达的原位杂交。结果:自发恢复呼吸侧膈神经活动中检测出的多数(15/20)C2把动物和44.06% + / - 2.38%时表示为一个百分比的noninjured动物的同侧的膈神经的活动。在急性研究中使用的最佳剂量,茶碱(15毫克/公斤)没有提高,而是出人意料地阻塞,恢复呼吸活动4中的5个动物测试。剂量的5毫克/公斤和2.5毫克/公斤,药物阻止恢复呼吸活动3的4和3中的5个动物测试,分别。 Quantitative analysis of adenosine A1 receptor mRNA expression did not reveal a significant difference between experimental animals and sham-operated animals. CONCLUSION: The blockade or attenuation of spontaneously recovered respiratory activity following theophylline administration cannot be attributed to changes in adenosine A1 receptors because there were no significant differences in adenosine A1 mRNA expression with sham-operated animals. Lack of alteration in A1 mRNA expression 4 months after cervical SCI suggests that A1 receptor plasticity is not activated by chronic injury. Obliteration of spontaneous recovery with theophylline most likely involves a separate unknown mechanism. These findings suggest that there may be a limited therapeutic window for the clinical application of theophylline in SCI patients with respiratory deficits. Theophylline may be more effective clinically in the acute phase of injury rather than in the chronic phase.