Dual specificity protein kinase CLK3

Details

Name
Dual specificity protein kinase CLK3
Synonyms
  • 2.7.12.1
  • CDC-like kinase 3
Gene Name
CLK3
Organism
Humans
Amino acid sequence
>lcl|BSEQ0007727|Dual specificity protein kinase CLK3 MPVLSARRRELADHAGSGRRSGPSPTARSGPHLSALRAQPARAAHLSGRGTYVRRDTAGG GPGQARPLGPPGTSLLGRGARRSGEGWCPGAFESGARAARPPSRVEPRLATAASREGAGL PRAEVAAGSGRGARSGEWGLAAAGAWETMHHCKRYRSPEPDPYLSYRWKRRRSYSREHEG RLRYPSRREPPPRRSRSRSHDRLPYQRRYRERRDSDTYRCEERSPSFGEDYYGPSRSRHR RRSRERGPYRTRKHAHHCHKRRTRSCSSASSRSQQSSKRSSRSVEDDKEGHLVCRIGDWL QERYEIVGNLGEGTFGKVVECLDHARGKSQVALKIIRNVGKYREAARLEINVLKKIKEKD KENKFLCVLMSDWFNFHGHMCIAFELLGKNTFEFLKENNFQPYPLPHVRHMAYQLCHALR FLHENQLTHTDLKPENILFVNSEFETLYNEHKSCEEKSVKNTSIRVADFGSATFDHEHHT TIVATRHYRPPEVILELGWAQPCDVWSIGCILFEYYRGFTLFQTHENREHLVMMEKILGP IPSHMIHRTRKQKYFYKGGLVWDENSSDGRYVKENCKPLKSYMLQDSLEHVQLFDLMRRM LEFDPAQRITLAEALLHPFFAGLTPEERSFHTSRNPSR
Number of residues
638
分子量
73514.365
Theoretical pI
10.37
GO Classification
Functions
ATP binding/poly(A) RNA binding/protein serine/threonine kinase activity/protein serine/threonine/tyrosine kinase activity/protein tyrosine kinase activity
Processes
protein autophosphorylation/protein phosphorylation/regulation of RNA splicing
Components
acrosomal vesicle/intermediate filament cytoskeleton/membrane/nuclear speck/nucleoplasm/nucleus
General Function
Protein tyrosine kinase activity
Specific Function
Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Phosphorylates SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Nucleus
Gene sequence
>lcl|BSEQ0012814|Dual specificity protein kinase CLK3 (CLK3) ATGCCCGTCCTCTCCGCGCGCAGGAGGGAGTTGGCGGACCACGCGGGGTCGGGGCGACGG AGCGGGCCCAGCCCCACGGCCAGGTCGGGGCCCCACCTCTCGGCTCTGAGAGCCCAGCCG GCCCGGGCCGCGCACCTGTCAGGTCGGGGGACCTACGTGCGCCGCGACACGGCGGGAGGC GGGCCGGGCCAGGCTCGTCCCCTCGGCCCTCCCGGAACTAGTCTCCTAGGCCGCGGCGCC CGCCGGAGCGGAGAGGGCTGGTGCCCCGGAGCCTTCGAGTCGGGGGCTAGAGCGGCCAGG CCTCCGAGCCGGGTCGAGCCGAGGCTGGCGACGGCTGCGTCACGCGAGGGGGCGGGGCTG CCACGGGCGGAGGTCGCAGCCGGAAGCGGAAGAGGCGCTCGGAGCGGGGAGTGGGGCCTA GCTGCAGCCGGAGCCTGGGAGACGATGCATCACTGTAAGCGATACCGCTCCCCTGAACCA GACCCGTACCTGAGCTACCGATGGAAGAGGAGGAGGTCCTACAGTCGGGAACATGAAGGG AGACTGCGATACCCGTCCCGAAGGGAGCCTCCCCCACGAAGATCTCGGTCCAGAAGCCAT GACCGCCTGCCCTACCAGAGGAGGTACCGGGAGCGCCGTGACAGCGATACATACCGGTGT GAAGAGCGGAGCCCATCCTTTGGAGAGGACTACTATGGACCTTCACGTTCTCGTCATCGT CGGCGATCGCGGGAGAGGGGGCCATACCGGACCCGCAAGCATGCCCACCACTGCCACAAA CGCCGCACCAGGTCTTGTAGCAGCGCCTCCTCGAGAAGCCAACAGAGCAGTAAGCGCAGC AGCCGGAGTGTGGAAGATGACAAGGAGGGTCACCTGGTGTGCCGGATCGGCGATTGGCTC CAAGAGCGATATGAGATTGTGGGGAACCTGGGTGAAGGCACCTTTGGCAAGGTGGTGGAG TGCTTGGACCATGCCAGAGGGAAGTCTCAGGTTGCCCTGAAGATCATCCGCAACGTGGGC AAGTACCGGGAGGCTGCCCGGCTAGAAATCAACGTGCTCAAAAAAATCAAGGAGAAGGAC AAAGAAAACAAGTTCCTGTGTGTCTTGATGTCTGACTGGTTCAACTTCCACGGTCACATG TGCATCGCCTTTGAGCTCCTGGGCAAGAACACCTTTGAGTTCCTGAAGGAGAATAACTTC CAGCCTTACCCCCTACCACATGTCCGGCACATGGCCTACCAGCTCTGCCACGCCCTTAGA TTTCTGCATGAGAATCAGCTGACCCATACAGACTTGAAACCAGAGAACATCCTGTTTGTG AATTCTGAGTTTGAAACCCTCTACAATGAGCACAAGAGCTGTGAGGAGAAGTCAGTGAAG AACACCAGCATCCGAGTGGCTGACTTTGGCAGTGCCACATTTGACCATGAGCACCACACC ACCATTGTGGCCACCCGTCACTATCGCCCGCCTGAGGTGATCCTTGAGCTGGGCTGGGCA CAGCCCTGTGACGTCTGGAGCATTGGCTGCATTCTCTTTGAGTACTACCGGGGCTTCACA CTCTTCCAGACCCACGAAAACCGAGAGCACCTGGTGATGATGGAGAAGATCCTAGGGCCC ATCCCATCACACATGATCCACCGTACCAGGAAGCAGAAATATTTCTACAAAGGGGGCCTA GTTTGGGATGAGAACAGCTCTGACGGCCGGTATGTGAAGGAGAACTGCAAACCTCTGAAG AGTTACATGCTCCAAGACTCCCTGGAGCACGTGCAGCTGTTTGACCTGATGAGGAGGATG TTAGAATTTGACCCTGCCCAGCGCATCACACTGGCCGAGGCCCTGCTGCACCCCTTCTTT GCTGGCCTGACCCCTGAGGAGCGGTCCTTCCACACCAGCCGCAACCCAAGCAGATGA
Chromosome Location
15
Locus
15抓起
External Identifiers
Resource Link
UniProtKB ID P49761
UniProtKB Entry Name CLK3_HUMAN
GenBank Protein ID 632970
GenBank Gene ID L29220
HGNC ID HGNC:2071
General References
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  4. Duncan PI, Stojdl DF, Marius RM, Scheit KH, Bell JC: The Clk2 and Clk3 dual-specificity protein kinases regulate the intranuclear distribution of SR proteins and influence pre-mRNA splicing. Exp Cell Res. 1998 Jun 15;241(2):300-8. [Article]
  5. Hillman RT, Green RE, Brenner SE: An unappreciated role for RNA surveillance. Genome Biol. 2004;5(2):R8. Epub 2004 Feb 2. [Article]
  6. Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M: Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell. 2006 Nov 3;127(3):635-48. [Article]
  7. Daub H, Olsen JV, Bairlein M, Gnad F, Oppermann FS, Korner R, Greff Z, Keri G, Stemmann O, Mann M: Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. Mol Cell. 2008 Aug 8;31(3):438-48. doi: 10.1016/j.molcel.2008.07.007. [Article]
  8. Dephoure N, Zhou C, Villen J, Beausoleil SA, Bakalarski CE, Elledge SJ, Gygi SP: A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A. 2008 Aug 5;105(31):10762-7. doi: 10.1073/pnas.0805139105. Epub 2008 Jul 31. [Article]
  9. Eisenreich A, Bogdanov VY, Zakrzewicz A, Pries A, Antoniak S, Poller W, Schultheiss HP, Rauch U: Cdc2-like kinases and DNA topoisomerase I regulate alternative splicing of tissue factor in human endothelial cells. Circ Res. 2009 Mar 13;104(5):589-99. doi: 10.1161/CIRCRESAHA.108.183905. Epub 2009 Jan 22. [Article]
  10. Mayya V, Lundgren DH, Hwang SI, Rezaul K, Wu L, Eng JK, Rodionov V, Han DK: Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. Sci Signal. 2009 Aug 18;2(84):ra46. doi: 10.1126/scisignal.2000007. [Article]
  11. Olsen JV, Vermeulen M, Santamaria A, Kumar C, Miller ML, Jensen LJ, Gnad F, Cox J, Jensen TS, Nigg EA, Brunak S, Mann M: Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal. 2010 Jan 12;3(104):ra3. doi: 10.1126/scisignal.2000475. [Article]
  12. Bian Y, Song C, Cheng K, Dong M, Wang F, Huang J, Sun D, Wang L, Ye M, Zou H: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics. 2014 Jan 16;96:253-62. doi: 10.1016/j.jprot.2013.11.014. Epub 2013 Nov 22. [Article]
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Drug Relations

Drug Relations
DrugBank ID Name Drug group Pharmacological action? Actions Details
DB07664 K-00546 experimental unknown Details
DB08691 ethyl 3-[(E)-2-amino-1-cyanoethenyl]-6,7-dichloro-1-methyl-1H-indole-2-carboxylate experimental unknown Details
DB12010 Fostamatinib approved, investigational unknown inhibitor Details